Biosynthesis of the enediyne antitumor antibiotic C-1027.
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Liu W, Christenson SD, Standage S, Shen B
Biosynthesis of the enediyne antitumor antibiotic C-1027.
Science. 2002 Aug 16;297(5584):1170-3.
- PubMed ID
- 12183628 [ View in PubMed]
- Abstract
C-1027 is a potent antitumor agent with a previously undescribed molecular architecture and mode of action. Cloning and characterization of the 85-kilobase C-1027 biosynthesis gene cluster from Streptomyces globisporus revealed (i) an iterative type I polyketide synthase that is distinct from any bacterial polyketide synthases known to date, (ii) a general polyketide pathway for the biosynthesis of both the 9- and 10-membered enediyne antibiotics, and (iii) a convergent biosynthetic strategy for the C-1027 chromophore from four building blocks. Manipulation of genes governing C-1027 biosynthesis allowed us to produce an enediyne compound in a predicted manner.