Molecular analysis of the recF gene of Escherichia coli.
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Blanar MA, Sandler SJ, Armengod ME, Ream LW, Clark AJ
Molecular analysis of the recF gene of Escherichia coli.
Proc Natl Acad Sci U S A. 1984 Aug;81(15):4622-6.
- PubMed ID
- 6379647 [ View in PubMed]
- Abstract
We analyzed the nucleotide sequence of a 1.325-kilobase region of wild-type Escherichia coli containing a functional recF gene and six Tn3 mutations that inactivate recF. The analysis shows a potentially translatable reading frame of 1071 nucleotides, which is interrupted by all six insertions. A protein of 40.5 kilodaltons would result from translation of the open reading frame, and a radioactive band of protein of an apparent molecular weight of approximately 40 kilodaltons was seen by the maxicell method using a recF+ plasmid. Putative truncated peptides were seen when two recF::Tn3 mutant plasmids were used. Differential expression of dnaN and recF from a common promoter was noted. recF332::Tn3 was transferred to the chromosome where, in hemizygous condition, it produced UV sensitivity indistinguishable from that produced by two presumed recF point mutations.