Evidence for a copper-coordinated histidine-tyrosine cross-link in the active site of cytochrome oxidase.
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Buse G, Soulimane T, Dewor M, Meyer HE, Bluggel M
Evidence for a copper-coordinated histidine-tyrosine cross-link in the active site of cytochrome oxidase.
Protein Sci. 1999 May;8(5):985-90.
- PubMed ID
- 10338009 [ View in PubMed]
- Abstract
Following hints from X-ray data (Ostermeier C et al., 1997, Proc Natl Acad Sci USA 94:10547-10553; Yoshikawa S et al., 1998, Science 280: 1723-1729), chemical evidence is presented from four distantly related cytochrome-c oxidases for the existence of a copperB-coordinated His240-Tyr244) cross-link at the O2-activating Heme Fea3-CuB center in the catalytic subunit 1 of the enzyme. The early evolutionary invention of this unusual structure may have prevented damaging *OH-radical release at e(-)-transfer to dioxygen and thus have enabled O2 respiration.