Protein D2 porin of the Pseudomonas aeruginosa outer membrane bears the protease activity.
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Yoshihara E, Gotoh N, Nishino T, Nakae T
Protein D2 porin of the Pseudomonas aeruginosa outer membrane bears the protease activity.
FEBS Lett. 1996 Sep 30;394(2):179-82.
- PubMed ID
- 8843159 [ View in PubMed]
- Abstract
We report here our discovery that protein D2 of the outer membrane of Pseudomonas aeruginosa is a novel porin bearing protease activity. Homogeneously purified protein D2 hydrolyzed several synthetic peptides according to the Michaelis-Menten kinetics. A specific serine protease inhibitor, diisopropyl fluorophosphate (DFP), inactivated the protease activity and [3H]DFP covalently labeled protein D2. We tested the effect of two monoclonal antibodies raised against protein D2 on the protease activity. One antibody lowered the protease activity to about 20%, while the other enhanced it to about 300% of that without antibody. In addition, the fractions derived from the outer membrane of the protein D2-deficient mutants showed negligible protease activity, whereas similarly fractionated outer membrane proteins of the protein D2-positive parent strain showed strong protease activity.