Molecular cloning and expression of the rat EAAT4 glutamate transporter subtype.

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Lin CL, Tzingounis AV, Jin L, Furuta A, Kavanaugh MP, Rothstein JD

Molecular cloning and expression of the rat EAAT4 glutamate transporter subtype.

Brain Res Mol Brain Res. 1998 Dec 10;63(1):174-9.

PubMed ID

9838098 [ View in PubMed

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Abstract

Glutamate transport is a primary mechanism for the synaptic inactivation of glutamate. Excitatory amino acid transporter 4 (EAAT4) is a novel glutamate transporter with properties of a ligand-gated chloride channel that was recently cloned from human brain. Here we report the cloning of rat EAAT4 (rEAAT4) cDNA from rat cerebellum. The nucleotide sequence of rEAAT4 was 88% identical to the human sequence, and the predicted peptide was 89% identical to the human protein. The transport activity encoded by rEAAT4 has high affinity for L-glutamate. In Xenopus laevis oocytes expressing rEAAT4, L-glutamate and other transporter substrates elicited a current predominantly carried by chloride ions. Like human EAAT4, the rEAAT4 mRNA was largely restricted to cerebellar Purkinje cells; the rEAAT4 protein was localized to Purkinje cell somas and dendrites.

DrugBank Data that Cites this Article

Drug Targets

Drug	Target	Kind	Organism	Pharmacological Action	Actions
Glutamic acid	Excitatory amino acid transporter 4	Protein	Humans	Unknown	Not Available	Details