High pressure liquid chromatographic determination of sulfanitran and dinsed in medicated feeds and premixes.

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Eaves KL, Colvin BM, Hanks AR, Bushway RJ

High pressure liquid chromatographic determination of sulfanitran and dinsed in medicated feeds and premixes.

J Assoc Off Anal Chem. 1977 Sep;60(5):1064-6.

PubMed ID
561053 [ View in PubMed
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Abstract

A simple reverse phase high pressure liquid chromatographic method is described for determining sulfanitran (acetyl-p-nitrophenylsulfanilamide) and Dinsed (dinitrodiphenylsulfonylethylenediamine) in a variety of feed premixes and formulations. Feed premixes are extracted with dimethylformamide, and formulated feeds are extracted with hot methanol. The extract is filtered through medium porosity paper and injected into a liquid chromatograph equipped with a 254 nm ultraviolet detector and a 30 cm column packed with muBondapak C18. The mobile phase is acetonitrile-water (45 + 55) at a flow rate of 1.0 ml/min. Chromatography was complete in 10 min and peak heights were used for quantitation. Comparison of analyses of commercial samples by this method and by the AOAC colorimetric method, 42.176-42.179, showed close agreement. Recovery of spiked feed samples ranged from 98 to 105%. Butynorate and roxarsone, 2 other drugs which are normally found in combination with sulfanitran and Dinsed, do not interfere.

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