Isolation of an ascitic oncodevelopmental protein exhibiting high sequence homology with calcium-binding protein MRP8.
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Nakai M, Ishikawa M, Hamada Y, Sugano S
Isolation of an ascitic oncodevelopmental protein exhibiting high sequence homology with calcium-binding protein MRP8.
Biol Chem Hoppe Seyler. 1994 Nov;375(11):789-92.
- PubMed ID
- 7695842 [ View in PubMed]
- Abstract
A human oncodevelopmental protein (ODP) with a molecular size of 28 kDa was isolated, employing malignant ascitic fluid of an advanced mixed mesodermal tumor of the uterus, by anti-ODP coupled Affi-Gel 10 column chromatography, followed by preparative PAGE. The final preparation obtained with this procedure gave a single band exhibiting reactivity with an anti-ODP antibody on analytical sodium dodecyl sulfate-PAGE. The sequence of the first 20 N-terminal amino acids of this protein is identical with those of both the cystic fibrosis protein and Ca-binding protein MRP8 except at position 17, for which no result was obtained. Sequence analysis suggested that the protein shows 90% sequence homology with both these proteins.