Cloning and pretranslational hormonal regulation of testosterone 16 alpha-hydroxylase (P-45016 alpha) in male rat liver.
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Strom A, Mode A, Zaphiropoulos P, Nilsson AG, Morgan E, Gustafsson JA
Cloning and pretranslational hormonal regulation of testosterone 16 alpha-hydroxylase (P-45016 alpha) in male rat liver.
Acta Endocrinol (Copenh). 1988 Jun;118(2):314-20.
- PubMed ID
- 2455430 [ View in PubMed]
- Abstract
cDNA clones for P-45016 alpha were isolated from a male liver lambda gt11 expression library using antibodies against P-45016 alpha. The clones encompassed 1633 and 1791 bp, respectively. The latter clone contained the whole coding sequence. The 20 deduced NH2-terminal amino acids were identical to those of P-450h and the cDNA sequence was in complete agreement with that of P-450 (M-1). Northern blots showed that P-45016 alpha in the rat liver is pretranslationally regulated by the growth hormone secretory pattern. Southern blots indicated that few genes belong to the same P-450 gene family as P-45016 alpha.