Human cytochrome P450: metabolism of testosterone by CYP3A4 and inhibition by ketoconazole.

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Usmani KA, Tang J

Human cytochrome P450: metabolism of testosterone by CYP3A4 and inhibition by ketoconazole.

Curr Protoc Toxicol. 2004 Jun;Chapter 4:Unit4.13. doi: 10.1002/0471140856.tx0413s20.

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20945304 [ View in PubMed

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Abstract

This unit describes methods for measuring CYP3A4 activity using testosterone as a specific substrate, and for measuring CYP3A4 inhibition using ketoconazole as a selective inhibitor of testosterone oxidation. CYP3A4 is one of the most important and most abundant drug-metabolizing CYP isoforms in human liver microsomes ( approximately 40% of total CYP), and it has the broadest substrate specificity. It is important to determine whether CYP3A4 is involved in its metabolism.

DrugBank Data that Cites this Article

Drug Enzymes

Drug	Enzyme	Kind	Organism	Pharmacological Action	Actions
Testosterone	Cytochrome P450 3A4	Protein	Humans	Unknown	Substrate Inducer	Details