Methylene blue inhibits hippocampal nitric oxide synthase activity in vivo.

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Volke V, Wegener G, Vasar E, Rosenberg R

Methylene blue inhibits hippocampal nitric oxide synthase activity in vivo.

Brain Res. 1999 May 1;826(2):303-5. doi: 10.1016/s0006-8993(99)01253-6.

PubMed ID

10224309 [ View in PubMed

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Abstract

The aim of the present study was to investigate the effect of methylene blue, a guanylate cyclase inhibitor, on the hippocampal nitric oxide synthase activity in vivo. We used a microdialysis-based technique of measuring conversion of [3H]l-arginine to [3H]l-citrulline in freely moving rats. The administration of methylene blue (0.1 and 1 mM) via the microdialysis probe caused a dose-dependent decrease in [3H]l-citrulline efflux comparable with the effect of unselective NOS inhibitor NG-nitro-L-arginine (2 mM). We conclude that methylene blue inhibits brain NOS activity in vivo and thus interferes with NO-cGMP cascade in different levels.

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Drug Targets

Drug	Target	Kind	Organism	Pharmacological Action	Actions
Methylene blue	Nitric oxide synthase, brain	Protein	Humans	Yes	Inhibitor	Details