A protein-coated magnetic beads as a tool for the rapid drug-protein binding study.
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Marszall MP, Bucinski A
A protein-coated magnetic beads as a tool for the rapid drug-protein binding study.
J Pharm Biomed Anal. 2010 Jul 8;52(3):420-4. doi: 10.1016/j.jpba.2009.06.023. Epub 2009 Jun 18.
- PubMed ID
- 19596536 [ View in PubMed]
- Abstract
A simple and fast method for the determination of the association constant (K(a)) of ligand to human serum albumin (HSA) has been developed by using human serum albumin-coated magnetic beads (HSA-MB). To date, magnetic beads (MB) have been increasingly used as a bioseparation tool, especially for DNA, RNA, protein, enzyme and cell isolation or purification. In this study, HSA-MB were used as a new tool to determine the affinity of known ligands to HSA. The K(a) for l-tryptofan, fenoprofen, ketoprofen, tolbutamide and warfarin obtained from Schathard analysis are consistent with previously reported values. The different K(a) values for ketoprofen after the acetylation of HSA-MB by preincubation with acetylosalicylic acid indicate that these beads can be successfully adapted in combined experiment. In addition, the HSA-MB experiment with phenytoin and valproic acid proved to be a simple method to examine drug displacement effect.
DrugBank Data that Cites this Article
- Drug Carriers
Drug Carrier Kind Organism Pharmacological Action Actions Tolbutamide Serum albumin Protein Humans UnknownNot Available Details