Hepatocellular toxicity of benzbromarone: effects on mitochondrial function and structure.
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Felser A, Lindinger PW, Schnell D, Kratschmar DV, Odermatt A, Mies S, Jeno P, Krahenbuhl S
Hepatocellular toxicity of benzbromarone: effects on mitochondrial function and structure.
Toxicology. 2014 Oct 3;324:136-46. doi: 10.1016/j.tox.2014.08.002. Epub 2014 Aug 7.
- PubMed ID
- 25108121 [ View in PubMed]
- Abstract
Benzbromarone is an uricosuric structurally related to amiodarone and a known mitochondrial toxicant. The aim of the current study was to improve our understanding in the molecular mechanisms of benzbromarone-associated hepatic mitochondrial toxicity. In HepG2 cells and primary human hepatocytes, ATP levels started to decrease in the presence of 25-50muM benzbromarone for 24-48h, whereas cytotoxicity was observed only at 100muM. In HepG2 cells, benzbromarone decreased the mitochondrial membrane potential starting at 50muM following incubation for 24h. Additionally, in HepG2 cells, 50muM benzbromarone for 24h induced mitochondrial uncoupling,and decreased mitochondrial ATP turnover and maximal respiration. This was accompanied by an increased lactate concentration in the cell culture supernatant, reflecting increased glycolysis as a compensatory mechanism to maintain cellular ATP. Investigation of the electron transport chain revealed a decreased activity of all relevant enzyme complexes. Furthermore, treatment with benzbromarone was associated with increased cellular ROS production, which could be located specifically to mitochondria. In HepG2 cells and in isolated mouse liver mitochondria, benzbromarone also reduced palmitic acid metabolism due to an inhibition of the long-chain acyl CoA synthetase. In HepG2 cells, benzbromarone disrupted the mitochondrial network, leading to mitochondrial fragmentation and a decreased mitochondrial volume per cell. Cell death occurred by both apoptosis and necrosis. The study demonstrates that benzbromarone not only affects the function of mitochondria in HepG2 cells and human hepatocytes, but is also associated with profound changes in mitochondrial structure which may be associated with apoptosis.
DrugBank Data that Cites this Article
- Pharmaco-transcriptomics
Drug Drug Groups Gene Gene ID Change Interaction Chromosome Benzbromarone Investigational Withdrawn CPT1A 1374 upregulated Benzbromarone results in increased expression of CPT1A mRNA 11q13.3 Benzbromarone Investigational Withdrawn SOD2 6648 upregulated Benzbromarone results in increased expression of SOD2 mRNA 6q25.3