Molecular cloning and expression of an IL-6 signal transducer, gp130.
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Hibi M, Murakami M, Saito M, Hirano T, Taga T, Kishimoto T
Molecular cloning and expression of an IL-6 signal transducer, gp130.
Cell. 1990 Dec 21;63(6):1149-57. doi: 10.1016/0092-8674(90)90411-7.
- PubMed ID
- 2261637 [ View in PubMed]
- Abstract
Interleukin-6 (IL-6) signal is transduced through a membrane glycoprotein, gp130, which associates with IL-6 receptor (IL-6-R). A cDNA encoding human gp130 has been cloned, revealing that it consists of 918 amino acids with a single transmembrane domain. The extracellular region comprises six units of a fibronectin type III module, and part of this region of approximately 200 amino acids has features typical of a cytokine receptor family. A cDNA-expressed gp130 showed no binding property to IL-6 or several other cytokines. Although a transfectant with an IL-6-R cDNA expressed mainly low affinity IL-6 binding sites, an increase in high affinity binding sites was observed after cotransfection with a gp130 cDNA. This confirmed that a gp130 is involved in the formation of high affinity IL-6 binding sites. A cloned gp130 could associate with a complex of IL-6 and soluble IL-6-R and transduce the growth signal when expressed in a murine IL-3-dependent cell line.