The inhibition characteristics of human placental glutathione S-transferase-pi by tricyclic antidepressants: amitriptyline and clomipramine.

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Dalmizrak O, Kulaksiz-Erkmen G, Ozer N

The inhibition characteristics of human placental glutathione S-transferase-pi by tricyclic antidepressants: amitriptyline and clomipramine.

Mol Cell Biochem. 2011 Sep;355(1-2):223-31. doi: 10.1007/s11010-011-0858-6. Epub 2011 May 13.

PubMed ID

21567209 [ View in PubMed

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Abstract

Tricyclic antidepressants (TCAs) are the non-selective amine re-uptake inhibitors, well absorbed from small intestine, cross the blood-brain barrier, distributed in the brain, and are bound to glutathione S-transferase-pi (GST-pi). TCAs can pass through placenta, accumulate in utero baby, and cause congenital malformations. Thus, the study of the interaction of GST-pi with antidepressants is crucial. In this study, the interaction of GST-pi with amitriptyline and clomipramine was investigated. The K (m) values for glutathione (GSH) and 1-chloro-2,4-dinitrobenzene (CDNB) were found to be 0.16 +/- 0.04 and 3.60 +/- 1.67 mM, respectively. The V (m) values were varying according to the fixed substrate; [CDNB] fixed, 53 +/- 3 and [GSH] fixed 182 +/- 63 U/mg protein. At variable [GSH] and variable [CDNB], the k (cat) values of 7.0 x 10(6) and 1.42 x 10(7) s(-1) and the k (cat)/K (m) values of 4.38 x 10(10) and 3.94 x 10(9 )M(-1 )s(-1) were obtained, respectively. At fixed [CDNB] and variable [GSH], amitriptyline (K (s) = 0.16 +/- 0.03 mM; alpha = 2.08; and K (i) = 1.75 +/- 0.37 mM) and clomipramine (K (s) = 0.24 +/- 0.05 mM; alpha = 1.57; and K (i) = 3.90 +/- 2.26 mM) showed linear mixed-type inhibition whereas when the varied substrate is CDNB, amitriptyline (K (i) = 4.90 +/- 0.68 mM) and clomipramine (K (i) = 3.37 +/- 0.39 mM) inhibition were noncompetitive. The inhibition of GST-pi by TCAs means the destruction of its protective role against toxic electrophiles. The effect of antidepressants on fetus will be much severe, thus, the antidepressant therapy of pregnant women should be done with caution.

DrugBank Data that Cites this Article

Drug Enzymes

Drug	Enzyme	Kind	Organism	Pharmacological Action	Actions
Amitriptyline	Glutathione S-transferases (Cytosolic) (Protein Group)	Protein group	Humans	Unknown	Inhibitor	Details