Characterization of the human small-ribosomal-subunit proteins by N-terminal and internal sequencing, and mass spectrometry.

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Vladimirov SN, Ivanov AV, Karpova GG, Musolyamov AK, Egorov TA, Thiede B, Wittmann-Liebold B, Otto A

Characterization of the human small-ribosomal-subunit proteins by N-terminal and internal sequencing, and mass spectrometry.

Eur J Biochem. 1996 Jul 1;239(1):144-9.

PubMed ID
8706699 [ View in PubMed
]
Abstract

Reverse-phase HPLC was used to fractionate 40S ribosomal proteins from human placenta. Application of a C4 reverse-phase column allowed us to obtain 27 well-resolved peaks. The protein composition of each chromatographic fraction was established by two-dimensional polyacrylamide gel electrophoresis and N-terminal sequencing. N-terminally blocked proteins were cleaved with endoproteinase Lys-C, and suitable peptides were sequenced. All sequences were compared with those of ribosomal proteins available from data bases. This allowed us to identify all proteins from the 40S human ribosomal subunit in the HPLC elution profile. By matrix-assisted laser-desorption ionization mass spectrometry the masses of the 40S proteins were determined and checked for the presence of post-translational modifications. For several proteins differences to the deduced sequences and the calculated masses were found to be due to post-translational modifications.

DrugBank Data that Cites this Article

Polypeptides
NameUniProt ID
Ferritin light chainP02792Details
40S ribosomal protein SAP08865Details
40S ribosomal protein S2P15880Details
40S ribosomal protein S13P62277Details
40S ribosomal protein S17P08708Details
40S ribosomal protein S18P62269Details
40S ribosomal protein S19P39019Details
40S ribosomal protein S28P62857Details
40S ribosomal protein S5P46782Details
40S ribosomal protein S6P62753Details
40S ribosomal protein S8P62241Details
40S ribosomal protein S9P46781Details