Gene expression profiling in the human hypothalamus-pituitary-adrenal axis and full-length cDNA cloning.

Article Details


Hu RM, Han ZG, Song HD, Peng YD, Huang QH, Ren SX, Gu YJ, Huang CH, Li YB, Jiang CL, Fu G, Zhang QH, Gu BW, Dai M, Mao YF, Gao GF, Rong R, Ye M, Zhou J, Xu SH, Gu J, Shi JX, Jin WR, Zhang CK, Wu TM, Huang GY, Chen Z, Chen MD, Chen JL

Gene expression profiling in the human hypothalamus-pituitary-adrenal axis and full-length cDNA cloning.

Proc Natl Acad Sci U S A. 2000 Aug 15;97(17):9543-8.

PubMed ID
10931946 [ View in PubMed

The primary neuroendocrine interface, hypothalamus and pituitary, together with adrenals, constitute the major axis responsible for the maintenance of homeostasis and the response to the perturbations in the environment. The gene expression profiling in the human hypothalamus-pituitary-adrenal axis was catalogued by generating a large amount of expressed sequence tags (ESTs), followed by bioinformatics analysis ( database). Totally, 25,973 sequences of good quality were obtained from 31,130 clones (83.4%) from cDNA libraries of the hypothalamus, pituitary, and adrenal glands. After eliminating 5,347 sequences corresponding to repetitive elements and mtDNA, 20,626 ESTs could be assembled into 9, 175 clusters (3,979, 3,074, and 4,116 clusters in hypothalamus, pituitary, and adrenal glands, respectively) when overlapping ESTs were integrated. Of these clusters, 2,777 (30.3%) corresponded to known genes, 4,165 (44.8%) to dbESTs, and 2,233 (24.3%) to novel ESTs. The gene expression profiles reflected well the functional characteristics of the three levels in the hypothalamus-pituitary-adrenal axis, because most of the 20 genes with highest expression showed statistical difference in terms of tissue distribution, including a group of tissue-specific functional markers. Meanwhile, some findings were made with regard to the physiology of the axis, and 200 full-length cDNAs of novel genes were cloned and sequenced. All of these data may contribute to the understanding of the neuroendocrine regulation of human life.

DrugBank Data that Cites this Article

NameUniProt ID
NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrialO75489Details
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 4-like 2Q9NRX3Details
NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrialP49821Details
NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7P17568Details
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13Q9P0J0Details
3-oxo-5-alpha-steroid 4-dehydrogenase 1P18405Details
UMP-CMP kinaseP30085Details
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12Q9UI09Details
Peroxiredoxin-5, mitochondrialP30044Details
Coatomer subunit gamma-1Q9Y678Details
S-formylglutathione hydrolaseP10768Details
Peroxisomal sarcosine oxidaseQ9P0Z9Details
ADP-ribosylation factor-like protein 5AQ9Y689Details
V-type proton ATPase catalytic subunit AP38606Details
Geranylgeranyl pyrophosphate synthaseO95749Details
Enolase-phosphatase E1Q9UHY7Details
Acyl-coenzyme A thioesterase 13Q9NPJ3Details
Diphthine methyl ester synthaseQ9H2P9Details
tRNA-splicing ligase RtcB homologQ9Y3I0Details
Transitional endoplasmic reticulum ATPaseP55072Details
Protein cereblonQ96SW2Details
Heterochromatin protein 1-binding protein 3Q5SSJ5Details
Eukaryotic translation initiation factor 2-alpha kinase 1Q9BQI3Details
NAD-dependent protein deacetylase sirtuin-2Q8IXJ6Details
V-type proton ATPase subunit DQ9Y5K8Details
V-type proton ATPase subunit HQ9UI12Details