Proteomic analysis of early melanosomes: identification of novel melanosomal proteins.
Article Details
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Basrur V, Yang F, Kushimoto T, Higashimoto Y, Yasumoto K, Valencia J, Muller J, Vieira WD, Watabe H, Shabanowitz J, Hearing VJ, Hunt DF, Appella E
Proteomic analysis of early melanosomes: identification of novel melanosomal proteins.
J Proteome Res. 2003 Jan-Feb;2(1):69-79.
- PubMed ID
- 12643545 [ View in PubMed]
- Abstract
Melanin is a heterogeneous biopolymer produced only by specific cells termed melanocytes, which synthesize and deposit the pigment in specialized membrane-bound organelles known as melanosomes. Although melanosomes have been suspected of being closely related to lysosomes and platelets, the total number of melanosomal proteins is still unknown. Thus far, six melanosome-specific proteins have been identified, and the challenge is to characterize the complete proteome of the melanosome to further understand its mechanism of biogenesis. In this report, we used mass spectrometry and subcellular fractionation to identify protein components of early melanosomes. Using this approach, we have identified all 6 of the known melanosome-specific proteins, 56 proteins that are shared with other organelles, and confirmed the presence of 6 novel melanosomal proteins using western blotting and by immunohistochemistry.
DrugBank Data that Cites this Article
- Polypeptides
Name UniProt ID Tyrosinase P14679 Details Gamma-glutamyl hydrolase Q92820 Details Cathepsin B P07858 Details Cathepsin D P07339 Details Calnexin P27824 Details Endoplasmic reticulum chaperone BiP P11021 Details Endoplasmin P14625 Details Protein disulfide-isomerase P07237 Details Transmembrane glycoprotein NMB Q14956 Details V-type proton ATPase subunit B, brain isoform P21281 Details Ras-related protein Rab-7a P51149 Details Protein disulfide-isomerase A3 P30101 Details V-type proton ATPase 116 kDa subunit a 1 Q93050 Details Melanocyte protein PMEL P40967 Details