Cloning and characterization of cDNA encoding human A-type endothelin receptor.

Article Details

Citation

Adachi M, Yang YY, Furuichi Y, Miyamoto C

Cloning and characterization of cDNA encoding human A-type endothelin receptor.

Biochem Biophys Res Commun. 1991 Nov 14;180(3):1265-72.

PubMed ID
1719979 [ View in PubMed
]
Abstract

A cDNA coding for the human A-type endothelin receptor (ETA) was cloned from a human placenta cDNA library. The cDNA contained the entire coding sequence for the 427 amino acid protein with a relative Mr of 48,722. The deduced amino acid sequence of the human ETA was, respectively, 94% and 93% homologous with the sequence of bovine ETA and rat ETA, but was only 64% homologous with that of the human ETB receptor. Upon expression in COS-1 cells, the human ETA receptor showed binding activity to ETA, with the highest selectivity to ET-1. Northern blot analysis showed that the mRNA of human placenta ETA consists of one species 5 kilo-nucleotides in length, and the same analysis for the uterus, testis, heart and adrenal gland of Cynomolgus monkey showed that the cognate mRNAs are widely distributed.

DrugBank Data that Cites this Article

Polypeptides
NameUniProt ID
Endothelin-1 receptorP25101Details