Toward a global characterization of the phosphoproteome in prostate cancer cells: identification of phosphoproteins in the LNCaP cell line.
Article Details
- CitationCopy to clipboard
Giorgianni F, Zhao Y, Desiderio DM, Beranova-Giorgianni S
Toward a global characterization of the phosphoproteome in prostate cancer cells: identification of phosphoproteins in the LNCaP cell line.
Electrophoresis. 2007 Jun;28(12):2027-34.
- PubMed ID
- 17487921 [ View in PubMed]
- Abstract
Protein phosphorylation plays a major role in most cell-signaling pathways in all eukaryotic cells. Disruptions in phosphorylation-mediated cell-signaling events are associated with various diseases, including cancer. Here, we applied a fully non-gel-based methodology to obtain an initial panel of phosphoproteins from the LNCaP human prostate cancer cell line. The analytical strategy involved enrichment of phosphopeptides by immobilized metal ion affinity chromatography, the use of POROS Oligo R3 to capture phosphopeptides that were not retained with a C18 packing, and gas-phase fractionation in the m/z dimension to extend the dynamic range of the LC-MS/MS analysis. In this pilot investigation, 137 phosphorylation sites in 81 phosphoproteins were identified. The characterized phosphoproteins include kinases, co-regulators of steroid receptors, and a number of cancer-related proteins.
DrugBank Data that Cites this Article
- Polypeptides
Name UniProt ID CTP synthase 1 P17812 Details Histone deacetylase 1 Q13547 Details DNA topoisomerase 2-beta Q02880 Details Prostaglandin E synthase 3 Q15185 Details cAMP-dependent protein kinase type II-alpha regulatory subunit P13861 Details Microtubule-associated protein 4 P27816 Details Cation-independent mannose-6-phosphate receptor P11717 Details Proteasome subunit alpha type-3 P25788 Details Membrane-associated progesterone receptor component 1 O00264 Details Hepatoma-derived growth factor P51858 Details Survival motor neuron protein B4DP61 Details Myosin-9 P35579 Details STE20/SPS1-related proline-alanine-rich protein kinase Q9UEW8 Details Phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit gamma O75747 Details Eukaryotic translation initiation factor 4E-binding protein 1 Q13541 Details