Toward a global characterization of the phosphoproteome in prostate cancer cells: identification of phosphoproteins in the LNCaP cell line.

Article Details

Citation

Giorgianni F, Zhao Y, Desiderio DM, Beranova-Giorgianni S

Toward a global characterization of the phosphoproteome in prostate cancer cells: identification of phosphoproteins in the LNCaP cell line.

Electrophoresis. 2007 Jun;28(12):2027-34.

PubMed ID
17487921 [ View in PubMed
]
Abstract

Protein phosphorylation plays a major role in most cell-signaling pathways in all eukaryotic cells. Disruptions in phosphorylation-mediated cell-signaling events are associated with various diseases, including cancer. Here, we applied a fully non-gel-based methodology to obtain an initial panel of phosphoproteins from the LNCaP human prostate cancer cell line. The analytical strategy involved enrichment of phosphopeptides by immobilized metal ion affinity chromatography, the use of POROS Oligo R3 to capture phosphopeptides that were not retained with a C18 packing, and gas-phase fractionation in the m/z dimension to extend the dynamic range of the LC-MS/MS analysis. In this pilot investigation, 137 phosphorylation sites in 81 phosphoproteins were identified. The characterized phosphoproteins include kinases, co-regulators of steroid receptors, and a number of cancer-related proteins.

DrugBank Data that Cites this Article

Polypeptides
NameUniProt ID
CTP synthase 1P17812Details
Histone deacetylase 1Q13547Details
DNA topoisomerase 2-betaQ02880Details
Prostaglandin E synthase 3Q15185Details
cAMP-dependent protein kinase type II-alpha regulatory subunitP13861Details
Microtubule-associated protein 4P27816Details
Cation-independent mannose-6-phosphate receptorP11717Details
Proteasome subunit alpha type-3P25788Details
Membrane-associated progesterone receptor component 1O00264Details
Hepatoma-derived growth factorP51858Details
Survival motor neuron proteinB4DP61Details
Myosin-9P35579Details
STE20/SPS1-related proline-alanine-rich protein kinaseQ9UEW8Details
Phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit gammaO75747Details