Human breast carcinoma cDNA encoding a galactoside-binding lectin homologous to mouse Mac-2 antigen.
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Oda Y, Leffler H, Sakakura Y, Kasai K, Barondes SH
Human breast carcinoma cDNA encoding a galactoside-binding lectin homologous to mouse Mac-2 antigen.
Gene. 1991 Mar 15;99(2):279-83.
- PubMed ID
- 2022338 [ View in PubMed]
- Abstract
A galactoside-binding lectin (Mr 29,000) has previously been identified in rat, mouse and human tissues. It is an abundant cell-surface component of inflammatory macrophages and their major non-integrin laminin-binding protein. It has also been found in the nucleus of other cell types. Here, we report the cloning and sequencing of a cDNA encoding the human galactoside-binding lectin from a breast carcinoma. The clone encodes a protein of 250 amino acids (aa) that is over 80% identical to its mouse and rat counterparts. The aa sequence has an N-terminal and a C-terminal, 'carbohydrate-binding', domain. The N-terminal domain consists of two parts. The first 41 aa are homologous to a transcription factor, i.e., the serum response factor. The adjacent part (aa 42-106) contains an unusual repeating element, that occurs seven times in human protein compared to nine times in rat and mouse. The C-terminal 'carbohydrate-binding' domain (aa 115-250) shows homology to L-14, another galactoside-binding lectin.