Isolation and structural analysis of the phospho-beta-galactosidase gene from Streptococcus lactis Z268.
Article Details
- CitationCopy to clipboard
Boizet B, Villeval D, Slos P, Novel M, Novel G, Mercenier A
Isolation and structural analysis of the phospho-beta-galactosidase gene from Streptococcus lactis Z268.
Gene. 1988;62(2):249-61.
- PubMed ID
- 3130294 [ View in PubMed]
- Abstract
A 4.4-kb XhoI fragment of Streptococcus lactis L13 (Z268) lactose plasmid pUCL13, containing the beta-D-phosphogalactoside galactohydrolase (P-beta Gal; EC 3.2.1.85)-coding gene has been cloned in Escherichia coli. Further subcloning and deletion of this fragment allowed localization of the P-beta Gal-coding gene (pbg) on a minimal 1.8-kb segment. Expression of P-beta Gal activity was constitutive and was not regulated by glucose in E. coli. The presence of P-beta Gal activity was correlated with the production of a 56.5-kDa protein in E. coli minicells. The nucleotide sequence of the cloned gene was determined and potential promoter structural elements were identified.