6-phospho-beta-galactosidase
Details
- Name
- 6-phospho-beta-galactosidase
- Synonyms
- 3.2.1.85
- Beta-D-phosphogalactoside galactohydrolase
- P-beta-Gal
- PBG
- PGALase
- Gene Name
- lacG
- Organism
- Lactococcus lactis subsp. lactis
- Amino acid sequence
>lcl|BSEQ0002848|6-phospho-beta-galactosidase MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWYTAEPASDFYHKYPVDL ELAEEYGVNGIRISIAWSRIFPTGYGEVNEKGVEFYHKLFAECHKRHVEPFVTLHHFDTP EALHSNGDFLNRENIEHFIDYAAFCFEEFPEVNYWTTFNEIGPIGDGQYLVGKFPPGIKY DLAKVFQSHHNMMVSHARAVKLYKDKGYKGEIGVVHALPTKYPYDPENPADVRAAELEDI IHNKFILDATYLGHYSDKTMEGVNHILAENGGELDLRDEDFQALDAAKDLNDFLGINYYM SDWMQAFDGETEIIHNGKGEKGSSKYQIKGVGRRVAPDYVPRTDWDWIIYPEGLYDQIMR VKNDYPNYKKIYITENGLGYKDEFVDNTVYDDGRIDYVKQHLEVLSDAIADGANVKGYFI WSLMDVFSWSNGYEKRYGLFYVDFDTQERYPKKSAHWYKKLAETQVIE
- Number of residues
- 468
- Molecular Weight
- 54072.005
- Theoretical pI
- 4.81
- GO Classification
- Functions6-phospho-beta-galactosidase activityProcesseslactose catabolic process via tagatose-6-phosphate
- General Function
- 6-phospho-beta-galactosidase activity
- Specific Function
- Not Available
- Pfam Domain Function
- Glyco_hydro_1 (PF00232)
- Transmembrane Regions
- Not Available
- Cellular Location
- Cytoplasmic
- Gene sequence
>lcl|BSEQ0002847|1407 bp ATGACTAAAACACTTCCTAAAGATTTTATTTTTGGTGGTGCTACAGCTGCTTATCAAGCA GAAGGTGCAACTCACACAGATGGTAAGGGCCCAGTAGCATGGGATAAGTATCTTGAAGAC AATTATTGGTATACAGCAGAACCTGCAAGTGATTTTTACCATAAATACCCAGTTGATTTA GAATTAGCTGAAGAATATGGTGTTAATGGTATTCGTATTTCTATTGCCTGGTCTCGTATT TTCCCAACGGGTTACGGTGAAGTAAATGAAAAAGGTGTTGAATTCTACCATAAACTATTT GCTGAATGCCACAAGCGTCATGTTGAACCATTTGTGACTTTGCATCATTTTGACACACCA GAAGCTCTTCATTCAAATGGAGATTTCTTAAACCGCGAAAATATAGAACACTTTATAGAT TATGCCGCTTTCTGTTTTGAAGAGTTTCCAGAAGTAAACTACTGGACAACTTTCAATGAA ATTGGCCCAATTGGTGATGGTCAATACTTGGTTGGTAAATTCCCTCCAGGCATTAAATAT GATCTTGCCAAAGTCTTCCAATCACACCACAACATGATGGTCTCTCATGCACGTGCTGTA AAATTGTATAAGGATAAAGGTTATAAAGGTGAAATTGGTGTTGTTCATGCTTTACCAACT AAATACCCTTATGATCCGGAAAATCCAGCAGATGTTCGTGCTGCTGAACTTGAAGACATC ATCCATAACAAATTTATCTTGGATGCAACCTATCTTGGGCACTATTCAGATAAAACGATG GAAGGTGTCAACCATATCCTAGCTGAGAATGGTGGAGAACTTGATCTTCGTGATGAAGAC TTCCAAGCTCTTGACGCAGCTAAAGATTTGAATGATTTCCTTGGTATCAACTATTACATG AGTGATTGGATGCAAGCTTTTGATGGTGAGACTGAAATCATTCACAATGGTAAGGGTGAA AAAGGAAGCTCTAAGTACCAAATTAAGGGTGTTGGTCGTCGAGTAGCTCCTGACTATGTT CCGCGCACAGACTGGGATTGGATTATTTATCCTGAAGGCTTGTATGACCAAATCATGCGA GTGAAAAATGATTATCCGAATTACAAGAAGATTTACATCACTGAAAACGGTCTCGGATAC AAAGATCAGTTTGTAGATAAAACTGTTTATGATGATGGTCGTATTGATTACGTGAAGCAA CACTTGGAAGTTTTATCAGACGCGATTGCGGATGGTGCAAATGTTAAAGGTTACTTCATT TGGTCACTTATGGACGTTTTCTCATGGTCAAATGGTTATGAAAAACGTTATGGATTGTTC TATGTAGACTTTGATACGCAAGAACGCTATCCTAAGAAATCAGCACATTGGTATAAGAAA TTAGCAGAAACTCAAGTGATAGAGTAA
- Chromosome Location
- Not Available
- Locus
- Not Available
- External Identifiers
Resource Link UniProtKB ID P11546 UniProtKB Entry Name LACG_LACLL GenBank Protein ID 149400 GenBank Gene ID M28357 - General References
- Boizet B, Villeval D, Slos P, Novel M, Novel G, Mercenier A: Isolation and structural analysis of the phospho-beta-galactosidase gene from Streptococcus lactis Z268. Gene. 1988;62(2):249-61. [Article]
- De Vos WM, Gasson MJ: Structure and expression of the Lactococcus lactis gene for phospho-beta-galactosidase (lacG) in Escherichia coli and L. lactis. J Gen Microbiol. 1989 Jul;135(7):1833-46. [Article]
- de Vos WM, Boerrigter I, van Rooyen RJ, Reiche B, Hengstenberg W: Characterization of the lactose-specific enzymes of the phosphotransferase system in Lactococcus lactis. J Biol Chem. 1990 Dec 25;265(36):22554-60. [Article]
- Huang DC, Novel M, Huang XF, Novel G: Nonidentity between plasmid and chromosomal copies of ISS1-like sequences in Lactococcus lactis subsp. lactis CNRZ270 and their possible role in chromosomal integration of plasmid genes. Gene. 1992 Sep 1;118(1):39-46. [Article]
- Wiesmann C, Beste G, Hengstenberg W, Schulz GE: The three-dimensional structure of 6-phospho-beta-galactosidase from Lactococcus lactis. Structure. 1995 Sep 15;3(9):961-8. [Article]
- Wiesmann C, Hengstenberg W, Schulz GE: Crystal structures and mechanism of 6-phospho-beta-galactosidase from Lactococcus lactis. J Mol Biol. 1997 Jun 27;269(5):851-60. [Article]