Protein complexes of the Escherichia coli cell envelope.

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Stenberg F, Chovanec P, Maslen SL, Robinson CV, Ilag LL, von Heijne G, Daley DO

Protein complexes of the Escherichia coli cell envelope.

J Biol Chem. 2005 Oct 14;280(41):34409-19. Epub 2005 Aug 3.

PubMed ID
16079137 [ View in PubMed
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Abstract

Protein complexes are an intrinsic aspect of life in the membrane. Knowing which proteins are assembled in these complexes is therefore essential to understanding protein function(s). Unfortunately, recent high throughput protein interaction studies have failed to deliver any significant information on proteins embedded in the membrane, and many membrane protein complexes remain ill defined. In this study, we have optimized the blue native-PAGE technique for the study of membrane protein complexes in the inner and outer membranes of Escherichia coli. In combination with second dimension SDS-PAGE and mass spectrometry, we have been able to identify 43 distinct protein complexes. In addition to a number of well characterized complexes, we have identified known and orphan proteins in novel oligomeric states. For two orphan proteins, YhcB and YjdB, our findings enable a tentative functional assignment. We propose that YhcB is a hitherto unidentified additional subunit of the cytochrome bd oxidase and that YjdB, which co-localizes with the ZipA protein, is involved in cell division. Our reference two-dimensional blue native-SDS-polyacrylamide gels will facilitate future studies of the assembly and composition of E. coli membrane protein complexes during different growth conditions and in different mutant backgrounds.

DrugBank Data that Cites this Article

Polypeptides
NameUniProt ID
Outer membrane protein FP02931Details
Long-chain fatty acid transport proteinP10384Details
Multidrug efflux pump subunit AcrBP31224Details
Outer membrane protein TolCP02930Details
ATP synthase subunit bP0ABA0Details
Outer membrane protein AP0A910Details
MaltoporinP02943Details
Succinate dehydrogenase flavoprotein subunitP0AC41Details
Succinate dehydrogenase iron-sulfur subunitP07014Details
Cell division protein ZipAP77173Details