Cloning, characterization and nucleotide sequences of two cDNAs encoding human pancreatic trypsinogens.
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Emi M, Nakamura Y, Ogawa M, Yamamoto T, Nishide T, Mori T, Matsubara K
Cloning, characterization and nucleotide sequences of two cDNAs encoding human pancreatic trypsinogens.
Gene. 1986;41(2-3):305-10.
- PubMed ID
- 3011602 [ View in PubMed]
- Abstract
Two cDNA clones encoding two major human trypsinogen isozymes were isolated from a human pancreatic cDNA library. The deduced amino acid (aa) sequences of the two trypsinogen precursors are found to have 89% sequence homology, and have the same number of aa (247), including 15 aa for a signal peptide and 8 aa for an activation peptide. Southern blot analysis of human genomic DNA using the cloned cDNA as a probe, revealed that the human trypsinogen genes constitute a multigene family of more than ten genes.