Molecular cloning and expression of human ribonuclease 4 cDNA.
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Seno M, Futami J, Tsushima Y, Akutagawa K, Kosaka M, Tada H, Yamada H
Molecular cloning and expression of human ribonuclease 4 cDNA.
Biochim Biophys Acta. 1995 Apr 26;1261(3):424-6.
- PubMed ID
- 7742370 [ View in PubMed]
- Abstract
A cDNA coding for human ribonuclease 4 was isolated from a pancreas cDNA library and sequenced. This cDNA (996 bp) includes an entire open reading frame encoding mature protein (119 aa) following signal peptide (28 aa). Expression of mature protein in Escherichia coli showed an apparent molecular mass of about 16 kDa, which was slightly lower than the mature form of human RNase 1, in SDS-PAGE.