Amidophosphoribosyltransferase
Details
- Name
- Amidophosphoribosyltransferase
- Kind
- protein
- Synonyms
- 2.4.2.14
- ATase
- Glutamine phosphoribosylpyrophosphate amidotransferase
- GPATase
- Gene Name
- purF
- UniProtKB Entry
- P00497Swiss-Prot
- Organism
- Bacillus subtilis (strain 168)
- NCBI Taxonomy ID
- 224308
- Amino acid sequence
>lcl|BSEQ0010962|Amidophosphoribosyltransferase MLAEIKGLNEECGVFGIWGHEEAPQITYYGLHSLQHRGQEGAGIVATDGEKLTAHKGQGL ITEVFQNGELSKVKGKGAIGHVRYATAGGGGYENVQPLLFRSQNNGSLALAHNGNLVNAT QLKQQLENQGSIFQTSSDTEVLAHLIKRSGHFTLKDQIKNSLSMLKGAYAFLIMTETEMI VALDPNGLRPLSIGMMGDAYVVASETCAFDVVGATYLREVEPGEMLIINDEGMKSERFSM NINRSICSMEYIYFSRPDSNIDGINVHSARKNLGKMLAQESAVEADVVTGVPDSSISAAI GYAEATGIPYELGLIKNRYVGRTFIQPSQALREQGVRMKLSAVRGVVEGKRVVMVDDSIV RGTTSRRIVTMLREAGATEVHVKISSPPIAHPCFYGIDTSTHEELIASSHSVEEIRQEIG ADTLSFLSVEGLLKGIGRKYDDSNCGQCLACFTGKYPTEIYQDTVLPHVKEAVLTK
- Number of residues
- 476
- Molecular Weight
- 51691.455
- Theoretical pI
- 6.28
- GO Classification
- Functions4 iron, 4 sulfur cluster binding / amidophosphoribosyltransferase activity / magnesium ion bindingProcesses'de novo' IMP biosynthetic process / glutamine metabolic process / nucleoside metabolic process / purine nucleobase biosynthetic process
- General Function
- Catalyzes the formation of phosphoribosylamine from phosphoribosylpyrophosphate (PRPP) and glutamine.
- Specific Function
- 4 iron, 4 sulfur cluster binding
- Pfam Domain Function
- Pribosyltran (PF00156)
- Signal Regions
- Not Available
- Transmembrane Regions
- Not Available
- Cellular Location
- Not Available
- Gene sequence
>lcl|BSEQ0010963|Amidophosphoribosyltransferase (purF) ATGCTTGCTGAAATCAAAGGCTTAAATGAAGAATGCGGCGTTTTTGGGATTTGGGGACAT GAAGAAGCCCCGCAAATCACGTATTACGGTCTCCACAGCCTTCAGCACCGAGGACAGGAG GGTGCTGGCATCGTAGCGACTGACGGTGAAAAGCTGACGGCTCACAAAGGCCAAGGTCTG ATCACTGAAGTATTTCAAAACGGCGAACTCAGCAAAGTAAAGGGAAAAGGCGCTATCGGG CACGTTCGGTACGCAACGGCTGGAGGCGGCGGATACGAAAATGTTCAGCCGCTCCTCTTC CGTTCCCAAAACAACGGCAGCCTGGCGCTTGCTCATAACGGAAATCTTGTCAACGCCACT CAGCTGAAGCAGCAGCTCGAAAATCAAGGGAGCATCTTTCAAACCTCTTCGGATACAGAG GTTTTGGCTCACCTGATCAAAAGAAGCGGACACTTCACGCTGAAGGATCAAATTAAAAAC TCGCTTTCTATGCTGAAAGGCGCCTACGCGTTCCTGATCATGACCGAAACAGAAATGATT GTCGCACTTGATCCAAACGGGCTGAGACCGCTATCCATCGGCATGATGGGCGACGCTTAT GTGGTCGCATCAGAAACATGCGCATTTGACGTCGTCGGCGCAACGTACCTTCGCGAGGTA GAGCCGGGAGAAATGCTGATCATTAATGATGAAGGCATGAAATCAGAGCGTTTTTCCATG AATATCAATCGTTCCATTTGCAGCATGGAGTACATTTATTTCTCCAGACCAGACAGCAAT ATTGACGGTATTAATGTGCACAGTGCCCGTAAAAACCTTGGGAAAATGCTGGCTCAGGAA TCCGCAGTTGAAGCTGACGTCGTAACCGGGGTTCCGGATTCCAGTATTTCAGCGGCGATC GGCTATGCAGAGGCAACAGGCATTCCGTATGAGCTTGGCTTAATCAAAAACCGTTATGTT GGCAGAACGTTTATTCAGCCGTCCCAGGCTCTGCGTGAGCAAGGCGTCAGAATGAAGCTG TCTGCGGTGCGCGGGGTTGTAGAAGGCAAACGCGTCGTGATGGTGGATGACTCTATCGTG CGAGGAACAACTAGCCGCCGGATTGTCACGATGCTAAGAGAAGCGGGTGCGACAGAGGTG CATGTGAAAATCAGTTCACCGCCGATCGCTCATCCGTGCTTTTACGGCATTGACACTTCC ACACATGAAGAACTGATCGCGTCTTCGCATTCTGTTGAAGAAATCCGTCAGGAAATCGGA GCCGATACCCTCTCATTTTTGAGTGTGGAAGGGCTGCTGAAAGGCATCGGCAGAAAATAC GATGACTCGAATTGCGGACAGTGTCTCGCTTGCTTTACAGGAAAATATCCGACTGAAATT TACCAGGATACAGTGCTTCCTCACGTAAAAGAAGCAGTATTAACCAAATAA
- Chromosome Location
- Not Available
- Locus
- Not Available
- External Identifiers
Resource Link UniProtKB ID P00497 UniProtKB Entry Name PUR1_BACSU GenBank Protein ID 143369 GenBank Gene ID J02732 PDB ID(s) 1AO0, 1GPH KEGG ID bsu:BSU06490 NCBI Gene ID 936046 - General References
- Makaroff CA, Zalkin H, Switzer RL, Vollmer SJ: Cloning of the Bacillus subtilis glutamine phosphoribosylpyrophosphate amidotransferase gene in Escherichia coli. Nucleotide sequence determination and properties of the plasmid-encoded enzyme. J Biol Chem. 1983 Sep 10;258(17):10586-93. [Article]
- Ebbole DJ, Zalkin H: Cloning and characterization of a 12-gene cluster from Bacillus subtilis encoding nine enzymes for de novo purine nucleotide synthesis. J Biol Chem. 1987 Jun 15;262(17):8274-87. [Article]
- Kunst F, Ogasawara N, Moszer I, Albertini AM, Alloni G, Azevedo V, Bertero MG, Bessieres P, Bolotin A, Borchert S, Borriss R, Boursier L, Brans A, Braun M, Brignell SC, Bron S, Brouillet S, Bruschi CV, Caldwell B, Capuano V, Carter NM, Choi SK, Cordani JJ, Connerton IF, Cummings NJ, Daniel RA, Denziot F, Devine KM, Dusterhoft A, Ehrlich SD, Emmerson PT, Entian KD, Errington J, Fabret C, Ferrari E, Foulger D, Fritz C, Fujita M, Fujita Y, Fuma S, Galizzi A, Galleron N, Ghim SY, Glaser P, Goffeau A, Golightly EJ, Grandi G, Guiseppi G, Guy BJ, Haga K, Haiech J, Harwood CR, Henaut A, Hilbert H, Holsappel S, Hosono S, Hullo MF, Itaya M, Jones L, Joris B, Karamata D, Kasahara Y, Klaerr-Blanchard M, Klein C, Kobayashi Y, Koetter P, Koningstein G, Krogh S, Kumano M, Kurita K, Lapidus A, Lardinois S, Lauber J, Lazarevic V, Lee SM, Levine A, Liu H, Masuda S, Mauel C, Medigue C, Medina N, Mellado RP, Mizuno M, Moestl D, Nakai S, Noback M, Noone D, O'Reilly M, Ogawa K, Ogiwara A, Oudega B, Park SH, Parro V, Pohl TM, Portelle D, Porwollik S, Prescott AM, Presecan E, Pujic P, Purnelle B, Rapoport G, Rey M, Reynolds S, Rieger M, Rivolta C, Rocha E, Roche B, Rose M, Sadaie Y, Sato T, Scanlan E, Schleich S, Schroeter R, Scoffone F, Sekiguchi J, Sekowska A, Seror SJ, Serror P, Shin BS, Soldo B, Sorokin A, Tacconi E, Takagi T, Takahashi H, Takemaru K, Takeuchi M, Tamakoshi A, Tanaka T, Terpstra P, Togoni A, Tosato V, Uchiyama S, Vandebol M, Vannier F, Vassarotti A, Viari A, Wambutt R, Wedler H, Weitzenegger T, Winters P, Wipat A, Yamamoto H, Yamane K, Yasumoto K, Yata K, Yoshida K, Yoshikawa HF, Zumstein E, Yoshikawa H, Danchin A: The complete genome sequence of the gram-positive bacterium Bacillus subtilis. Nature. 1997 Nov 20;390(6657):249-56. [Article]
- Barbe V, Cruveiller S, Kunst F, Lenoble P, Meurice G, Sekowska A, Vallenet D, Wang T, Moszer I, Medigue C, Danchin A: From a consortium sequence to a unified sequence: the Bacillus subtilis 168 reference genome a decade later. Microbiology. 2009 Jun;155(Pt 6):1758-75. doi: 10.1099/mic.0.027839-0. Epub 2009 Apr 21. [Article]
- Wong JY, Bernlohr DA, Turnbough CL, Switzer RL: Purification and properties of glutamine phosphoribosylpyrophosphate amidotransferase from Bacillus subtilis. Biochemistry. 1981 Sep 29;20(20):5669-74. [Article]
- Vollmer SJ, Switzer RL, Hermodson MA, Bower SG, Zalkin H: The glutamine-utilizing site of Bacillus subtilis glutamine phosphoribosylpyrophosphate amidotransferase. J Biol Chem. 1983 Sep 10;258(17):10582-5. [Article]
- Mantsala P, Zalkin H: Glutamine amidotransferase function. Replacement of the active-site cysteine in glutamine phosphoribosylpyrophosphate amidotransferase by site-directed mutagenesis. J Biol Chem. 1984 Nov 25;259(22):14230-6. [Article]
- Makaroff CA, Paluh JL, Zalkin H: Mutagenesis of ligands to the [4 Fe-4S] center of Bacillus subtilis glutamine phosphoribosylpyrophosphate amidotransferase. J Biol Chem. 1986 Aug 25;261(24):11416-23. [Article]
- Smith JL, Zaluzec EJ, Wery JP, Niu L, Switzer RL, Zalkin H, Satow Y: Structure of the allosteric regulatory enzyme of purine biosynthesis. Science. 1994 Jun 3;264(5164):1427-33. [Article]
- Chen S, Tomchick DR, Wolle D, Hu P, Smith JL, Switzer RL, Zalkin H: Mechanism of the synergistic end-product regulation of Bacillus subtilis glutamine phosphoribosylpyrophosphate amidotransferase by nucleotides. Biochemistry. 1997 Sep 2;36(35):10718-26. [Article]
Associated Data
- Drug Relations
Drug Drug group Pharmacological action? Type Actions Details Guanosine-5'-Monophosphate experimental unknown target Details