Holliday junction ATP-dependent DNA helicase RuvB
Details
- Name
- Holliday junction ATP-dependent DNA helicase RuvB
- Kind
- protein
- Synonyms
- 3.6.4.12
- Gene Name
- ruvB
- UniProtKB Entry
- Q56313Swiss-Prot
- Organism
- Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
- NCBI Taxonomy ID
- 243274
- Amino acid sequence
>lcl|BSEQ0011070|Holliday junction ATP-dependent DNA helicase RuvB MSEFLTPERTVYDSGVQFLRPKSLDEFIGQENVKKKLSLALEAAKMRGEVLDHVLLAGPP GLGKTTLAHIIASELQTNIHVTSGPVLVKQGDMAAILTSLERGDVLFIDEIHRLNKAVEE LLYSAIEDFQIDIMIGKGPSAKSIRIDIQPFTLVGATTRSGLLSSPLRSRFGIILELDFY TVKELKEIIKRAASLMDVEIEDAAAEMIAKRSRGTPRIAIRLTKRVRDMLTVVKADRINT DIVLKTMEVLNIDDEGLDEFDRKILKTIIEIYRGGPVGLNALAASLGVEADTLSEVYEPY LLQAGFLARTPRGRIVTEKAYKHLKYEVPENRLF
- Number of residues
- 334
- Molecular Weight
- 37155.775
- Theoretical pI
- 6.39
- GO Classification
- FunctionsATP binding / DNA binding / four-way junction helicase activityProcessesDNA recombination / DNA repair / SOS response
- General Function
- The RuvA-RuvB-RuvC complex processes Holliday junction (HJ) DNA during genetic recombination and DNA repair, while the RuvA-RuvB complex plays an important role in the rescue of blocked DNA replication forks via replication fork reversal (RFR). RuvA specifically binds to HJ cruciform DNA, conferring on it an open structure. The RuvB hexamer acts as an ATP-dependent pump, pulling dsDNA into and through the RuvAB complex. RuvB forms 2 homohexamers on either side of HJ DNA bound by 1 or 2 RuvA tetramers; 4 subunits per hexamer contact DNA at a time. Coordinated motions by a converter formed by DNA-disengaged RuvB subunits stimulates ATP hydrolysis and nucleotide exchange. Immobilization of the converter enables RuvB to convert the ATP-contained energy into a lever motion, pulling 2 nucleotides of DNA out of the RuvA tetramer per ATP hydrolyzed, thus driving DNA branch migration. The RuvB motors rotate together with the DNA substrate, which together with the progressing nucleotide cycle form the mechanistic basis for DNA recombination by continuous HJ branch migration. Branch migration allows RuvC to scan DNA until it finds its consensus sequence, where it cleaves and resolves cruciform DNA.
- Specific Function
- ATP binding
- Pfam Domain Function
- Signal Regions
- Not Available
- Transmembrane Regions
- Not Available
- Cellular Location
- Cytoplasmic
- Gene sequence
>lcl|BSEQ0011071|Holliday junction ATP-dependent DNA helicase RuvB (ruvB) GTGAGTGAATTTCTCACACCTGAAAGGACCGTTTACGACTCTGGTGTACAGTTTCTAAGG CCCAAAAGCCTCGATGAATTCATTGGTCAGGAAAACGTGAAAAAGAAACTCTCCCTCGCT CTCGAAGCCGCGAAGATGAGGGGAGAAGTGCTCGATCATGTCCTCCTCGCAGGACCACCG GGACTCGGAAAGACGACCCTTGCACACATAATCGCCAGCGAACTCCAGACGAACATCCAC GTTACGAGCGGACCGGTTCTTGTGAAACAGGGAGATATGGCCGCTATCCTCACAAGTCTG GAACGGGGAGACGTTCTTTTCATAGACGAAATACACCGATTGAACAAGGCAGTGGAAGAG CTTCTTTACTCTGCCATCGAAGACTTCCAGATAGACATCATGATCGGAAAGGGCCCGAGT GCAAAGTCCATTAGGATAGACATCCAGCCTTTTACGCTCGTTGGAGCCACGACGAGAAGT GGTCTTTTGAGTTCTCCTCTCAGAAGCAGGTTTGGTATCATCCTCGAACTGGACTTCTAC ACTGTGAAAGAACTGAAGGAAATCATAAAAAGAGCGGCCAGCTTGATGGACGTTGAGATA GAAGACGCAGCAGCAGAGATGATCGCGAAAAGATCGAGAGGCACACCGAGGATCGCTATA AGACTCACGAAGAGAGTGAGGGACATGCTCACGGTGGTAAAGGCAGACAGAATCAATACC GATATCGTTTTGAAGACCATGGAAGTTCTGAACATAGACGACGAGGGACTTGATGAGTTC GACAGGAAGATCCTGAAGACGATCATAGAGATTTACAGGGGAGGCCCCGTCGGATTGAAC GCCCTCGCCGCTTCACTCGGTGTAGAAGCGGACACCCTGAGCGAAGTTTATGAACCTTAC CTCCTCCAGGCAGGATTCCTCGCCAGAACTCCCAGAGGAAGGATCGTCACTGAAAAGGCT TACAAACACCTGAAGTACGAAGTCCCGGAAAACCGTCTTTTCTGA
- Chromosome Location
- Not Available
- Locus
- Not Available
- External Identifiers
Resource Link UniProtKB ID Q56313 UniProtKB Entry Name RUVB_THEMA GenBank Protein ID 1063668 GenBank Gene ID U38840 PDB ID(s) 1IN4, 1IN5, 1IN6, 1IN7, 1IN8, 1J7K KEGG ID tma:TM1730 NCBI Gene ID 897204 - General References
- Tong J, Wetmur JG: Cloning, sequencing, and expression of ruvB and characterization of RuvB proteins from two distantly related thermophilic eubacteria. J Bacteriol. 1996 May;178(9):2695-700. [Article]
- Nelson KE, Clayton RA, Gill SR, Gwinn ML, Dodson RJ, Haft DH, Hickey EK, Peterson JD, Nelson WC, Ketchum KA, McDonald L, Utterback TR, Malek JA, Linher KD, Garrett MM, Stewart AM, Cotton MD, Pratt MS, Phillips CA, Richardson D, Heidelberg J, Sutton GG, Fleischmann RD, Eisen JA, White O, Salzberg SL, Smith HO, Venter JC, Fraser CM: Evidence for lateral gene transfer between Archaea and bacteria from genome sequence of Thermotoga maritima. Nature. 1999 May 27;399(6734):323-9. [Article]
Associated Data
- Drug Relations
Drug Drug group Pharmacological action? Type Actions Details Hexane-1,6-Diol experimental unknown target Details