5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase

Details

Name
5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase
Kind
protein
Synonyms
  • 3.2.2.9
  • 5'-methylthioadenosine nucleosidase
  • AdoHcy nucleosidase
  • MTA nucleosidase
  • MTA/SAH nucleosidase
  • S-adenosylhomocysteine nucleosidase
  • SAH nucleosidase
  • SRH nucleosidase
Gene Name
mtnN
UniProtKB Entry
P0AF14Swiss-Prot
Organism
Escherichia coli O157:H7
NCBI Taxonomy ID
83334
Amino acid sequence
>lcl|BSEQ0012809|5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase
MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVALLKSGIGKVAAALGAT
LLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADVTAFGYEYGQLPGCPAGFK
ADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAKIRHNFPQAIAVEMEATAIAH
VCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQSSLMVESLVQKLAHG
Number of residues
232
Molecular Weight
24353.725
Theoretical pI
4.9
GO Classification
Functions
adenosylhomocysteine nucleosidase activity / methylthioadenosine nucleosidase activity
Processes
L-methionine salvage from methylthioadenosine / nucleoside catabolic process
General Function
Catalyzes the irreversible cleavage of the glycosidic bond in both 5'-methylthioadenosine (MTA) and S-adenosylhomocysteine (SAH/AdoHcy) to adenine and the corresponding thioribose, 5'-methylthioribose and S-ribosylhomocysteine, respectively. Also cleaves 5'-deoxyadenosine, a toxic by-product of radical S-adenosylmethionine (SAM) enzymes, into 5-deoxyribose and adenine. Thus, is required for in vivo function of the radical SAM enzymes biotin synthase and lipoic acid synthase, that are inhibited by 5'-deoxyadenosine accumulation.
Specific Function
adenosylhomocysteine nucleosidase activity
Pfam Domain Function
Signal Regions
Not Available
Transmembrane Regions
Not Available
Cellular Location
Cytoplasmic
Gene sequence
>lcl|BSEQ0012810|5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (mtnN)
ATGAAAATCGGCATCATTGGTGCAATGGAAGAAGAAGTTACGCTGCTGCGTGACAAAATC
GAAAACCGTCAAACTATCAGTCTGGGCGGTTGCGAAATCTATACCGGCCAACTGAATGGA
ACCGAGGTTGCGCTTCTGAAATCGGGCATCGGTAAAGTCGCTGCGGCGCTGGGTGCCACT
CTGCTGTTGGAACACTGCAAGCCAGATGTGATTATTAACACCGGTTCTGCCGGTGGCCTG
GCACCAACGCTGAAAGTGGGCGATATCGTTGTCTCGGACGAAGCGCGTTATCACGACGCG
GATGTCACGGCATTTGGTTATGAATACGGTCAGTTACCAGGCTGTCCGGCAGGCTTTAAA
GCTGACGATAAACTGATCGCTGCCGCTGAGGCCTGCATTGCCGAACTGAATCTTAACGCT
GTACGTGGCCTGATTGTTAGCGGCGACGCTTTCATCAACGGTTCTGTTGGTCTGGCGAAA
ATCCGCCACAACTTCCCACAGGCCATTGCTGTAGAGATGGAAGCGACGGCAATCGCCCAT
GTCTGCCACAATTTCAACGTCCCGTTTGTTGTCGTACGCGCCATCTCCGACGTGGCCGAT
CAACAGTCTCATCTTAGCTTCGATGAGTTCCTGGCTGTTGCCGCTAAACAGTCCAGCCTG
ATGGTTGAGTCACTAGTGCAGAAACTGGCACATGGCTAA
Chromosome Location
Not Available
Locus
Not Available
External Identifiers
ResourceLink
UniProtKB IDP0AF14
UniProtKB Entry NameMTNN_ECO57
GenBank Protein ID12512676
GenBank Gene IDAE005174
PDB ID(s)3DF9
KEGG IDece:Z0170
NCBI Gene ID913817
General References
  1. Perna NT, Plunkett G 3rd, Burland V, Mau B, Glasner JD, Rose DJ, Mayhew GF, Evans PS, Gregor J, Kirkpatrick HA, Posfai G, Hackett J, Klink S, Boutin A, Shao Y, Miller L, Grotbeck EJ, Davis NW, Lim A, Dimalanta ET, Potamousis KD, Apodaca J, Anantharaman TS, Lin J, Yen G, Schwartz DC, Welch RA, Blattner FR: Genome sequence of enterohaemorrhagic Escherichia coli O157:H7. Nature. 2001 Jan 25;409(6819):529-33. [Article]
  2. Hayashi T, Makino K, Ohnishi M, Kurokawa K, Ishii K, Yokoyama K, Han CG, Ohtsubo E, Nakayama K, Murata T, Tanaka M, Tobe T, Iida T, Takami H, Honda T, Sasakawa C, Ogasawara N, Yasunaga T, Kuhara S, Shiba T, Hattori M, Shinagawa H: Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12. DNA Res. 2001 Feb 28;8(1):11-22. [Article]

Associated Data

Drug Relations
DrugDrug groupPharmacological action?TypeActionsDetails
(3R,4S)-1-[(4-amino-5H-pyrrolo[3,2-d]pyrimidin-7-yl)methyl]-4-[(benzylsulfanyl)methyl]pyrrolidin-3-olexperimentalunknowntargetDetails