5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase
Details
- Name
- 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase
- Kind
- protein
- Synonyms
- 3.2.2.9
- 5'-methylthioadenosine nucleosidase
- AdoHcy nucleosidase
- MTA nucleosidase
- MTA/SAH nucleosidase
- S-adenosylhomocysteine nucleosidase
- SAH nucleosidase
- SRH nucleosidase
- Gene Name
- mtnN
- UniProtKB Entry
- P0AF14Swiss-Prot
- Organism
- Escherichia coli O157:H7
- NCBI Taxonomy ID
- 83334
- Amino acid sequence
>lcl|BSEQ0012809|5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVALLKSGIGKVAAALGAT LLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADVTAFGYEYGQLPGCPAGFK ADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAKIRHNFPQAIAVEMEATAIAH VCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQSSLMVESLVQKLAHG
- Number of residues
- 232
- Molecular Weight
- 24353.725
- Theoretical pI
- 4.9
- GO Classification
- Functionsadenosylhomocysteine nucleosidase activity / methylthioadenosine nucleosidase activityProcessesL-methionine salvage from methylthioadenosine / nucleoside catabolic process
- General Function
- Catalyzes the irreversible cleavage of the glycosidic bond in both 5'-methylthioadenosine (MTA) and S-adenosylhomocysteine (SAH/AdoHcy) to adenine and the corresponding thioribose, 5'-methylthioribose and S-ribosylhomocysteine, respectively. Also cleaves 5'-deoxyadenosine, a toxic by-product of radical S-adenosylmethionine (SAM) enzymes, into 5-deoxyribose and adenine. Thus, is required for in vivo function of the radical SAM enzymes biotin synthase and lipoic acid synthase, that are inhibited by 5'-deoxyadenosine accumulation.
- Specific Function
- adenosylhomocysteine nucleosidase activity
- Pfam Domain Function
- PNP_UDP_1 (PF01048)
- Signal Regions
- Not Available
- Transmembrane Regions
- Not Available
- Cellular Location
- Cytoplasmic
- Gene sequence
>lcl|BSEQ0012810|5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (mtnN) ATGAAAATCGGCATCATTGGTGCAATGGAAGAAGAAGTTACGCTGCTGCGTGACAAAATC GAAAACCGTCAAACTATCAGTCTGGGCGGTTGCGAAATCTATACCGGCCAACTGAATGGA ACCGAGGTTGCGCTTCTGAAATCGGGCATCGGTAAAGTCGCTGCGGCGCTGGGTGCCACT CTGCTGTTGGAACACTGCAAGCCAGATGTGATTATTAACACCGGTTCTGCCGGTGGCCTG GCACCAACGCTGAAAGTGGGCGATATCGTTGTCTCGGACGAAGCGCGTTATCACGACGCG GATGTCACGGCATTTGGTTATGAATACGGTCAGTTACCAGGCTGTCCGGCAGGCTTTAAA GCTGACGATAAACTGATCGCTGCCGCTGAGGCCTGCATTGCCGAACTGAATCTTAACGCT GTACGTGGCCTGATTGTTAGCGGCGACGCTTTCATCAACGGTTCTGTTGGTCTGGCGAAA ATCCGCCACAACTTCCCACAGGCCATTGCTGTAGAGATGGAAGCGACGGCAATCGCCCAT GTCTGCCACAATTTCAACGTCCCGTTTGTTGTCGTACGCGCCATCTCCGACGTGGCCGAT CAACAGTCTCATCTTAGCTTCGATGAGTTCCTGGCTGTTGCCGCTAAACAGTCCAGCCTG ATGGTTGAGTCACTAGTGCAGAAACTGGCACATGGCTAA
- Chromosome Location
- Not Available
- Locus
- Not Available
- External Identifiers
Resource Link UniProtKB ID P0AF14 UniProtKB Entry Name MTNN_ECO57 GenBank Protein ID 12512676 GenBank Gene ID AE005174 PDB ID(s) 3DF9 KEGG ID ece:Z0170 NCBI Gene ID 913817 - General References
- Perna NT, Plunkett G 3rd, Burland V, Mau B, Glasner JD, Rose DJ, Mayhew GF, Evans PS, Gregor J, Kirkpatrick HA, Posfai G, Hackett J, Klink S, Boutin A, Shao Y, Miller L, Grotbeck EJ, Davis NW, Lim A, Dimalanta ET, Potamousis KD, Apodaca J, Anantharaman TS, Lin J, Yen G, Schwartz DC, Welch RA, Blattner FR: Genome sequence of enterohaemorrhagic Escherichia coli O157:H7. Nature. 2001 Jan 25;409(6819):529-33. [Article]
- Hayashi T, Makino K, Ohnishi M, Kurokawa K, Ishii K, Yokoyama K, Han CG, Ohtsubo E, Nakayama K, Murata T, Tanaka M, Tobe T, Iida T, Takami H, Honda T, Sasakawa C, Ogasawara N, Yasunaga T, Kuhara S, Shiba T, Hattori M, Shinagawa H: Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12. DNA Res. 2001 Feb 28;8(1):11-22. [Article]
Associated Data
- Drug Relations
Drug Drug group Pharmacological action? Type Actions Details (3R,4S)-1-[(4-amino-5H-pyrrolo[3,2-d]pyrimidin-7-yl)methyl]-4-[(benzylsulfanyl)methyl]pyrrolidin-3-ol experimental unknown target Details