Lipase EstA
Details
- Name
- Lipase EstA
- Kind
- protein
- Synonyms
- lip
- lipA
- Lipase A
- Triacylglycerol lipase
- Gene Name
- estA
- UniProtKB Entry
- P37957Swiss-Prot
- Organism
- Bacillus subtilis (strain 168)
- NCBI Taxonomy ID
- 224308
- Amino acid sequence
>lcl|BSEQ0013022|Lipase EstA MKFVKRRIIALVTILMLSVTSLFALQPSAKAAEHNPVVMVHGIGGASFNFAGIKSYLVSQ GWSRDKLYAVDFWDKTGTNYNNGPVLSRFVQKVLDETGAKKVDIVAHSMGGANTLYYIKN LDGGNKVANVVTLGGANRLTTGKALPGTDPNQKILYTSIYSSADMIVMNYLSRLDGARNV QIHGVGHIGLLYSSQVNSLIKEGLNGGGQNTN
- Number of residues
- 212
- Molecular Weight
- 22791.075
- Theoretical pI
- 10.22
- GO Classification
- Functionstriglyceride lipase activityProcesseslipid catabolic processComponentsextracellular region
- General Function
- Active toward triacylglycerides with a preference for esters with C8:0 acyl groups; barely active on C18:1 or C18:4 substrates. Active against p-nitrophenylesters with fatty acid chain lengths from C6 to C18.
- Specific Function
- lipase activity
- Pfam Domain Function
- Lipase_2 (PF01674)
- Signal Regions
- 1-31
- Transmembrane Regions
- Not Available
- Cellular Location
- Secreted
- Gene sequence
>lcl|BSEQ0013023|Lipase EstA (estA) ATGAAATTTGTAAAAAGAAGGATCATTGCACTTGTAACAATTTTGATGCTGTCTGTTACA TCGCTGTTTGCGTTGCAGCCGTCAGCAAAAGCCGCTGAACACAATCCAGTCGTTATGGTT CACGGTATTGGAGGGGCATCATTCAATTTTGCGGGAATTAAGAGCTATCTCGTATCTCAG GGCTGGTCGCGGGACAAGCTGTATGCAGTTGATTTTTGGGACAAGACAGGCACAAATTAT AACAATGGACCGGTATTATCACGATTTGTGCAAAAGGTTTTAGATGAAACGGGTGCGAAA AAAGTGGATATTGTCGCTCACAGCATGGGGGGCGCGAACACACTTTACTACATAAAAAAT CTGGACGGCGGAAATAAAGTTGCAAACGTCGTGACGCTTGGCGGCGCGAACCGTTTGACG ACAGGCAAGGCGCTTCCGGGAACAGATCCAAATCAAAAGATTTTATACACATCCATTTAC AGCAGTGCCGATATGATTGTCATGAATTACTTATCAAGATTAGATGGTGCTAGAAACGTT CAAATCCATGGCGTTGGACACATCGGCCTTCTGTACAGCAGCCAAGTCAACAGCCTGATT AAAGAAGGGCTGAACGGCGGGGGCCAGAATACGAATTAA
- Chromosome Location
- Not Available
- Locus
- Not Available
- External Identifiers
Resource Link UniProtKB ID P37957 UniProtKB Entry Name ESTA_BACSU GenBank Protein ID 143154 GenBank Gene ID M74010 PDB ID(s) 1I6W, 1ISP, 1R4Z, 1R50, 1T2N, 1T4M, 2QXT, 2QXU, 3D2A, 3D2B, 3D2C, 3QMM, 3QZU, 5CT6 KEGG ID bsu:BSU02700 NCBI Gene ID 938389 - General References
- Dartois V, Baulard A, Schanck K, Colson C: Cloning, nucleotide sequence and expression in Escherichia coli of a lipase gene from Bacillus subtilis 168. Biochim Biophys Acta. 1992 Jul 15;1131(3):253-60. [Article]
- Kumano M, Tamakoshi A, Yamane K: A 32 kb nucleotide sequence from the region of the lincomycin-resistance gene (22 degrees-25 degrees) of the Bacillus subtilis chromosome and identification of the site of the lin-2 mutation. Microbiology. 1997 Aug;143 ( Pt 8):2775-82. [Article]
- Kunst F, Ogasawara N, Moszer I, Albertini AM, Alloni G, Azevedo V, Bertero MG, Bessieres P, Bolotin A, Borchert S, Borriss R, Boursier L, Brans A, Braun M, Brignell SC, Bron S, Brouillet S, Bruschi CV, Caldwell B, Capuano V, Carter NM, Choi SK, Cordani JJ, Connerton IF, Cummings NJ, Daniel RA, Denziot F, Devine KM, Dusterhoft A, Ehrlich SD, Emmerson PT, Entian KD, Errington J, Fabret C, Ferrari E, Foulger D, Fritz C, Fujita M, Fujita Y, Fuma S, Galizzi A, Galleron N, Ghim SY, Glaser P, Goffeau A, Golightly EJ, Grandi G, Guiseppi G, Guy BJ, Haga K, Haiech J, Harwood CR, Henaut A, Hilbert H, Holsappel S, Hosono S, Hullo MF, Itaya M, Jones L, Joris B, Karamata D, Kasahara Y, Klaerr-Blanchard M, Klein C, Kobayashi Y, Koetter P, Koningstein G, Krogh S, Kumano M, Kurita K, Lapidus A, Lardinois S, Lauber J, Lazarevic V, Lee SM, Levine A, Liu H, Masuda S, Mauel C, Medigue C, Medina N, Mellado RP, Mizuno M, Moestl D, Nakai S, Noback M, Noone D, O'Reilly M, Ogawa K, Ogiwara A, Oudega B, Park SH, Parro V, Pohl TM, Portelle D, Porwollik S, Prescott AM, Presecan E, Pujic P, Purnelle B, Rapoport G, Rey M, Reynolds S, Rieger M, Rivolta C, Rocha E, Roche B, Rose M, Sadaie Y, Sato T, Scanlan E, Schleich S, Schroeter R, Scoffone F, Sekiguchi J, Sekowska A, Seror SJ, Serror P, Shin BS, Soldo B, Sorokin A, Tacconi E, Takagi T, Takahashi H, Takemaru K, Takeuchi M, Tamakoshi A, Tanaka T, Terpstra P, Togoni A, Tosato V, Uchiyama S, Vandebol M, Vannier F, Vassarotti A, Viari A, Wambutt R, Wedler H, Weitzenegger T, Winters P, Wipat A, Yamamoto H, Yamane K, Yasumoto K, Yata K, Yoshida K, Yoshikawa HF, Zumstein E, Yoshikawa H, Danchin A: The complete genome sequence of the gram-positive bacterium Bacillus subtilis. Nature. 1997 Nov 20;390(6657):249-56. [Article]
- Lesuisse E, Schanck K, Colson C: Purification and preliminary characterization of the extracellular lipase of Bacillus subtilis 168, an extremely basic pH-tolerant enzyme. Eur J Biochem. 1993 Aug 15;216(1):155-60. [Article]
- Eggert T, van Pouderoyen G, Dijkstra BW, Jaeger KE: Lipolytic enzymes LipA and LipB from Bacillus subtilis differ in regulation of gene expression, biochemical properties, and three-dimensional structure. FEBS Lett. 2001 Aug 3;502(3):89-92. [Article]
- van Pouderoyen G, Eggert T, Jaeger KE, Dijkstra BW: The crystal structure of Bacillus subtilis lipase: a minimal alpha/beta hydrolase fold enzyme. J Mol Biol. 2001 May 25;309(1):215-26. [Article]
- Kawasaki K, Kondo H, Suzuki M, Ohgiya S, Tsuda S: Alternate conformations observed in catalytic serine of Bacillus subtilis lipase determined at 1.3 A resolution. Acta Crystallogr D Biol Crystallogr. 2002 Jul;58(Pt 7):1168-74. Epub 2002 Jun 20. [Article]
Associated Data
- Drug Relations
Drug Drug group Pharmacological action? Type Actions Details [(4R)-2,2-dimethyl-1,3-dioxolan-4-yl]methyl hydrogen hex-5-enylphosphonate experimental unknown target Details [(4S)-2,2-dimethyl-1,3-dioxolan-4-yl]methyl hydrogen hex-5-enylphosphonate experimental unknown target Details