Cocaine esterase

Details

Name
Cocaine esterase
Kind
protein
Synonyms
  • 3.1.1.84
  • Carboxylesterase 2
  • CE-2
  • hCE-2
  • ICE
  • Methylumbelliferyl-acetate deacetylase 2
Gene Name
CES2
UniProtKB Entry
O00748Swiss-Prot
Organism
Humans
NCBI Taxonomy ID
9606
Amino acid sequence
>lcl|BSEQ0008563|Cocaine esterase
MRLHRLRARLSAVACGLLLLLVRGQGQDSASPIRTTHTGQVLGSLVHVKGANAGVQTFLG
IPFAKPPLGPLRFAPPEPPESWSGVRDGTTHPAMCLQDLTAVESEFLSQFNMTFPSDSMS
EDCLYLSIYTPAHSHEGSNLPVMVWIHGGALVFGMASLYDGSMLAALENVVVVIIQYRLG
VLGFFSTGDKHATGNWGYLDQVAALRWVQQNIAHFGGNPDRVTIFGESAGGTSVSSLVVS
PISQGLFHGAIMESGVALLPGLIASSADVISTVVANLSACDQVDSEALVGCLRGKSKEEI
LAINKPFKMIPGVVDGVFLPRHPQELLASADFQPVPSIVGVNNNEFGWLIPKVMRIYDTQ
KEMDREASQAALQKMLTLLMLPPTFGDLLREEYIGDNGDPQTLQAQFQEMMADSMFVIPA
LQVAHFQCSRAPVYFYEFQHQPSWLKNIRPPHMKADHGDELPFVFRSFFGGNYIKFTEEE
EQLSRKMMKYWANFARNGNPNGEGLPHWPLFDQEEQYLQLNLQPAVGRALKAHRLQFWKK
ALPQKIQELEEPEERHTEL
Number of residues
559
Molecular Weight
61806.41
Theoretical pI
Not Available
GO Classification
Functions
carboxylesterase activity
Processes
prostaglandin metabolic process
Components
intracellular membrane-bounded organelle
General Function
Involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs (PubMed:9169443). Shows high catalytic efficiency for hydrolysis of cocaine, 4-methylumbelliferyl acetate, heroin and 6-monoacetylmorphine (PubMed:9169443). Hydrolyzes aspirin, substrates with large alcohol group and small acyl group and endogenous lipids such as triacylglycerol (PubMed:28677105). Converts monoacylglycerides to free fatty acids and glycerol. Hydrolyzes of 2-arachidonoylglycerol and prostaglandins (PubMed:21049984)
Specific Function
Carboxylesterase activity
Pfam Domain Function
Signal Regions
1-26
Transmembrane Regions
Not Available
Cellular Location
Endoplasmic reticulum lumen
Gene sequence
>lcl|BSEQ0013276|Cocaine esterase (CES2)
ATGACTGCTCAGTCCCGCTCTCCTACCACACCCACCTTTCCCGGCCCAAGCCAGCGCACC
CCGCTGACTCCCTGCCCAGTCCAAACTCCAAGGCTGGGCAAGGCACTGATCCACTGCTGG
ACAGACCCGGGGCAGCCTCTGGGTGAACAGCAGCGTGTCCGCCGGCAGCGAACCGAGACC
AGCGAGCCGACCATGCGGCTGCACAGACTTCGTGCGCGGCTGAGCGCGGTGGCCTGTGGG
CTTCTGCTGCTTCTTGTCCGGGGCCAGGGCCAGGACTCAGCCAGTCCCATCCGGACCACA
CACACGGGGCAGGTGCTGGGGAGTCTTGTCCATGTGAAGGGCGCCAATGCCGGGGTCCAA
ACCTTCCTGGGAATTCCATTTGCCAAGCCACCTCTAGGTCCGCTGCGATTTGCACCCCCT
GAGCCCCCTGAATCTTGGAGTGGTGTGAGGGATGGAACCACCCATCCGGCCATGTGTCTA
CAGGACCTCACCGCAGTGGAGTCAGAGTTTCTTAGCCAGTTCAACATGACCTTCCCTTCC
GACTCCATGTCTGAGGACTGCCTGTACCTCAGCATCTACACGCCGGCCCATAGCCATGAA
GGCTCTAACCTGCCGGTGATGGTGTGGATCCACGGTGGTGCGCTTGTTTTTGGCATGGCT
TCCTTGTATGATGGTTCCATGCTGGCTGCCTTGGAGAACGTGGTGGTGGTCATCATCCAG
TACCGCCTGGGTGTCCTGGGCTTCTTCAGCACTGGAGACAAGCACGCAACCGGCAACTGG
GGCTACCTGGACCAAGTGGCTGCACTACGCTGGGTCCAGCAGAATATCGCCCACTTTGGA
GGCAACCCTGACCGTGTCACCATTTTTGGCGAGTCTGCGGGTGGCACGAGTGTGTCTTCG
CTTGTTGTGTCCCCCATATCCCAAGGACTCTTCCACGGAGCCATCATGGAGAGTGGCGTG
GCCCTCCTGCCCGGCCTCATTGCCAGCTCAGCTGATGTCATCTCCACGGTGGTGGCCAAC
CTGTCTGCCTGTGACCAAGTTGACTCTGAGGCCCTGGTGGGCTGCCTGCGGGGCAAGAGT
AAAGAGGAGATTCTTGCAATTAACAAGCCTTTCAAGATGATCCCCGGAGTGGTGGATGGG
GTCTTCCTGCCCAGGCACCCCCAGGAGCTGCTGGCCTCTGCCGACTTTCAGCCTGTCCCT
AGCATTGTTGGTGTCAACAACAATGAATTCGGCTGGCTCATCCCCAAGGTCATGAGGATC
TATGATACCCAGAAGGAAATGGACAGAGAGGCCTCCCAGGCTGCTCTGCAGAAAATGTTA
ACGCTGCTGATGTTGCCTCCTACATTTGGTGACCTGCTGAGGGAGGAGTACATTGGGGAC
AATGGGGATCCCCAGACCCTCCAAGCGCAGTTCCAGGAGATGATGGCGGACTCCATGTTT
GTGATCCCTGCACTCCAAGTAGCACATTTTCAGTGTTCCCGGGCCCCTGTGTACTTCTAC
GAGTTCCAGCATCAGCCCAGCTGGCTCAAGAACATCAGGCCACCGCACATGAAGGCAGAC
CATGGTGATGAGCTTCCTTTTGTTTTCAGAAGTTTCTTTGGGGGCAACTACATTAAATTC
ACTGAGGAAGAGGAGCAGCTAAGCAGGAAGATGATGAAGTACTGGGCCAACTTTGCGAGA
AATGGGAACCCCAATGGCGAGGGTCTGCCACACTGGCCGCTGTTCGACCAGGAGGAGCAA
TACCTGCAGCTGAACCTACAGCCTGCGGTGGGCCGGGCTCTGAAGGCCCACAGGCTCCAG
TTCTGGAAGAAGGCGCTGCCCCAAAAGATCCAGGAGCTCGAGGAGCCTGAAGAGAGACAC
ACAGAGCTGTAG
Chromosome Location
16
Locus
16q22.1
External Identifiers
ResourceLink
UniProtKB IDO00748
UniProtKB Entry NameEST2_HUMAN
GeneCard IDCES2
HGNC IDHGNC:1864
KEGG IDhsa:8824
NCBI Gene ID8824
General References
  1. Schwer H, Langmann T, Daig R, Becker A, Aslanidis C, Schmitz G: Molecular cloning and characterization of a novel putative carboxylesterase, present in human intestine and liver. Biochem Biophys Res Commun. 1997 Apr 7;233(1):117-20. [Article]
  2. Pindel EV, Kedishvili NY, Abraham TL, Brzezinski MR, Zhang J, Dean RA, Bosron WF: Purification and cloning of a broad substrate specificity human liver carboxylesterase that catalyzes the hydrolysis of cocaine and heroin. J Biol Chem. 1997 Jun 6;272(23):14769-75. [Article]
  3. Ota T, Suzuki Y, Nishikawa T, Otsuki T, Sugiyama T, Irie R, Wakamatsu A, Hayashi K, Sato H, Nagai K, Kimura K, Makita H, Sekine M, Obayashi M, Nishi T, Shibahara T, Tanaka T, Ishii S, Yamamoto J, Saito K, Kawai Y, Isono Y, Nakamura Y, Nagahari K, Murakami K, Yasuda T, Iwayanagi T, Wagatsuma M, Shiratori A, Sudo H, Hosoiri T, Kaku Y, Kodaira H, Kondo H, Sugawara M, Takahashi M, Kanda K, Yokoi T, Furuya T, Kikkawa E, Omura Y, Abe K, Kamihara K, Katsuta N, Sato K, Tanikawa M, Yamazaki M, Ninomiya K, Ishibashi T, Yamashita H, Murakawa K, Fujimori K, Tanai H, Kimata M, Watanabe M, Hiraoka S, Chiba Y, Ishida S, Ono Y, Takiguchi S, Watanabe S, Yosida M, Hotuta T, Kusano J, Kanehori K, Takahashi-Fujii A, Hara H, Tanase TO, Nomura Y, Togiya S, Komai F, Hara R, Takeuchi K, Arita M, Imose N, Musashino K, Yuuki H, Oshima A, Sasaki N, Aotsuka S, Yoshikawa Y, Matsunawa H, Ichihara T, Shiohata N, Sano S, Moriya S, Momiyama H, Satoh N, Takami S, Terashima Y, Suzuki O, Nakagawa S, Senoh A, Mizoguchi H, Goto Y, Shimizu F, Wakebe H, Hishigaki H, Watanabe T, Sugiyama A, Takemoto M, Kawakami B, Yamazaki M, Watanabe K, Kumagai A, Itakura S, Fukuzumi Y, Fujimori Y, Komiyama M, Tashiro H, Tanigami A, Fujiwara T, Ono T, Yamada K, Fujii Y, Ozaki K, Hirao M, Ohmori Y, Kawabata A, Hikiji T, Kobatake N, Inagaki H, Ikema Y, Okamoto S, Okitani R, Kawakami T, Noguchi S, Itoh T, Shigeta K, Senba T, Matsumura K, Nakajima Y, Mizuno T, Morinaga M, Sasaki M, Togashi T, Oyama M, Hata H, Watanabe M, Komatsu T, Mizushima-Sugano J, Satoh T, Shirai Y, Takahashi Y, Nakagawa K, Okumura K, Nagase T, Nomura N, Kikuchi H, Masuho Y, Yamashita R, Nakai K, Yada T, Nakamura Y, Ohara O, Isogai T, Sugano S: Complete sequencing and characterization of 21,243 full-length human cDNAs. Nat Genet. 2004 Jan;36(1):40-5. Epub 2003 Dec 21. [Article]
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  9. Bian Y, Song C, Cheng K, Dong M, Wang F, Huang J, Sun D, Wang L, Ye M, Zou H: An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome. J Proteomics. 2014 Jan 16;96:253-62. doi: 10.1016/j.jprot.2013.11.014. Epub 2013 Nov 22. [Article]
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Associated Data

Drug Relations
DrugDrug groupPharmacological action?TypeActionsDetails
PrasugrelapprovedunknownenzymesubstrateDetails
Dabigatran etexilateapprovedunknownenzymesubstrateDetails
Filgotinibapproved, investigationalunknownenzymemetabolizerDetails
VicagrelinvestigationalunknownenzymesubstrateDetails
SimvastatinapprovedunknownenzymeinhibitorDetails
Deucravacitinibapproved, investigationalunknownenzymesubstrateDetails
Telotristat ethylapproved, investigationalunknownenzymesubstrateDetails