Interleukin-4

Details

Name
Interleukin-4
Synonyms
  • B-cell stimulatory factor 1
  • Binetrakin
  • BSF-1
  • IL-4
  • Lymphocyte stimulatory factor 1
  • Pitrakinra
Gene Name
IL4
Organism
Humans
Amino acid sequence
>lcl|BSEQ0006792|Interleukin-4
MGLTSQLLPPLFFLLACAGNFVHGHKCDITLQEIIKTLNSLTEQKTLCTELTVTDIFAAS
KNTTEKETFCRAATVLRQFYSHHEKDTRCLGATAQQFHRHKQLIRFLKRLDRNLWGLAGL
NSCPVKEANQSTLENFLERLKTIMREKYSKCSS
Number of residues
153
Molecular Weight
17492.09
Theoretical pI
9.16
GO Classification
Functions
cytokine activity / growth factor activity / interleukin-4 receptor binding
Processes
B cell costimulation / B cell differentiation / cellular defense response / cellular response to mercury ion / chemotaxis / cholesterol metabolic process / connective tissue growth factor biosynthetic process / defense response to protozoan / dendritic cell differentiation / extrinsic apoptotic signaling pathway in absence of ligand / female pregnancy / immune response / innate immune response in mucosa / microglial cell activation / myeloid dendritic cell differentiation / negative regulation of acute inflammatory response / negative regulation of apoptotic process / negative regulation of chronic inflammatory response / negative regulation of complement-dependent cytotoxicity / negative regulation of endothelial cell apoptotic process / negative regulation of epithelial cell migration / negative regulation of extrinsic apoptotic signaling pathway / negative regulation of macrophage activation / negative regulation of nitric oxide biosynthetic process / negative regulation of osteoclast differentiation / negative regulation of T-helper 17 cell differentiation / negative regulation of transcription, DNA-templated / negative regulation of white fat cell proliferation / positive regulation of activated T cell proliferation / positive regulation of B cell proliferation / positive regulation of chemokine biosynthetic process / positive regulation of defense response to virus by host / positive regulation of eosinophil chemotaxis / positive regulation of interleukin-10 production / positive regulation of interleukin-13 production / positive regulation of isotype switching to IgE isotypes / positive regulation of isotype switching to IgG isotypes / positive regulation of mast cell degranulation / positive regulation of MHC class II biosynthetic process / positive regulation of mononuclear cell migration / positive regulation of myoblast fusion / positive regulation of sequence-specific DNA binding transcription factor activity / positive regulation of T cell differentiation / positive regulation of T cell proliferation / positive regulation of transcription from RNA polymerase II promoter / positive regulation of transcription, DNA-templated / positive regulation of tyrosine phosphorylation of Stat5 protein / regulation of immune response / regulation of isotype switching / regulation of phosphorylation / regulation of proton transport / response to cytokine / response to drug / response to ethanol / response to nutrient / response to organic cyclic compound / retina development in camera-type eye / T-helper 1 cell lineage commitment / T-helper 2 cell cytokine production / T-helper 2 cell differentiation / type 2 immune response
Components
external side of plasma membrane / extracellular space
General Function
Interleukin-4 receptor binding
Specific Function
Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes.
Pfam Domain Function
Transmembrane Regions
Not Available
Cellular Location
Secreted
Gene sequence
>lcl|BSEQ0019434|Interleukin-4 (IL4)
ATGGGTCTCACCTCCCAACTGCTTCCCCCTCTGTTCTTCCTGCTAGCATGTGCCGGCAAC
TTTGTCCACGGACACAAGTGCGATATCACCTTACAGGAGATCATCAAAACTTTGAACAGC
CTCACAGAGCAGAAGACTCTGTGCACCGAGTTGACCGTAACAGACATCTTTGCTGCCTCC
AAGAACACAACTGAGAAGGAAACCTTCTGCAGGGCTGCGACTGTGCTCCGGCAGTTCTAC
AGCCACCATGAGAAGGACACTCGCTGCCTGGGTGCGACTGCACAGCAGTTCCACAGGCAC
AAGCAGCTGATCCGATTCCTGAAACGGCTCGACAGGAACCTCTGGGGCCTGGCGGGCTTG
AATTCCTGTCCTGTGAAGGAAGCCAACCAGAGTACGTTGGAAAACTTCTTGGAAAGGCTA
AAGACGATCATGAGAGAGAAATATTCAAAGTGTTCGAGCTGA
Chromosome Location
5
Locus
Not Available
External Identifiers
ResourceLink
UniProtKB IDP05112
UniProtKB Entry NameIL4_HUMAN
GenBank Gene IDX06750
GenAtlas IDIL4
HGNC IDHGNC:6014
General References
  1. Yokota T, Otsuka T, Mosmann T, Banchereau J, DeFrance T, Blanchard D, De Vries JE, Lee F, Arai K: Isolation and characterization of a human interleukin cDNA clone, homologous to mouse B-cell stimulatory factor 1, that expresses B-cell- and T-cell-stimulating activities. Proc Natl Acad Sci U S A. 1986 Aug;83(16):5894-8. [Article]
  2. Arai N, Nomura D, Villaret D, DeWaal Malefijt R, Seiki M, Yoshida M, Minoshima S, Fukuyama R, Maekawa M, Kudoh J, et al.: Complete nucleotide sequence of the chromosomal gene for human IL-4 and its expression. J Immunol. 1989 Jan 1;142(1):274-82. [Article]
  3. Klein SC, Golverdingen JG, Bouwens AG, Tilanus MG, de Weger RA: An alternatively spliced interleukin 4 form in lymphoid cells. Immunogenetics. 1995;41(1):57. [Article]
  4. Gerhard DS, Wagner L, Feingold EA, Shenmen CM, Grouse LH, Schuler G, Klein SL, Old S, Rasooly R, Good P, Guyer M, Peck AM, Derge JG, Lipman D, Collins FS, Jang W, Sherry S, Feolo M, Misquitta L, Lee E, Rotmistrovsky K, Greenhut SF, Schaefer CF, Buetow K, Bonner TI, Haussler D, Kent J, Kiekhaus M, Furey T, Brent M, Prange C, Schreiber K, Shapiro N, Bhat NK, Hopkins RF, Hsie F, Driscoll T, Soares MB, Casavant TL, Scheetz TE, Brown-stein MJ, Usdin TB, Toshiyuki S, Carninci P, Piao Y, Dudekula DB, Ko MS, Kawakami K, Suzuki Y, Sugano S, Gruber CE, Smith MR, Simmons B, Moore T, Waterman R, Johnson SL, Ruan Y, Wei CL, Mathavan S, Gunaratne PH, Wu J, Garcia AM, Hulyk SW, Fuh E, Yuan Y, Sneed A, Kowis C, Hodgson A, Muzny DM, McPherson J, Gibbs RA, Fahey J, Helton E, Ketteman M, Madan A, Rodrigues S, Sanchez A, Whiting M, Madari A, Young AC, Wetherby KD, Granite SJ, Kwong PN, Brinkley CP, Pearson RL, Bouffard GG, Blakesly RW, Green ED, Dickson MC, Rodriguez AC, Grimwood J, Schmutz J, Myers RM, Butterfield YS, Griffith M, Griffith OL, Krzywinski MI, Liao N, Morin R, Palmquist D, Petrescu AS, Skalska U, Smailus DE, Stott JM, Schnerch A, Schein JE, Jones SJ, Holt RA, Baross A, Marra MA, Clifton S, Makowski KA, Bosak S, Malek J: The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Genome Res. 2004 Oct;14(10B):2121-7. [Article]
  5. Eder A, Krafft-Czepa H, Krammer PH: The 5' region of the human interleukin 4 gene: structure and potential regulatory elements. Nucleic Acids Res. 1988 Jan 25;16(2):772. [Article]
  6. Carr C, Aykent S, Kimack NM, Levine AD: Disulfide assignments in recombinant mouse and human interleukin 4. Biochemistry. 1991 Feb 12;30(6):1515-23. [Article]
  7. Walter MR, Cook WJ, Zhao BG, Cameron RP Jr, Ealick SE, Walter RL Jr, Reichert P, Nagabhushan TL, Trotta PP, Bugg CE: Crystal structure of recombinant human interleukin-4. J Biol Chem. 1992 Oct 5;267(28):20371-6. [Article]
  8. Wlodawer A, Pavlovsky A, Gustchina A: Crystal structure of human recombinant interleukin-4 at 2.25 A resolution. FEBS Lett. 1992 Aug 31;309(1):59-64. [Article]
  9. Hage T, Sebald W, Reinemer P: Crystal structure of the interleukin-4/receptor alpha chain complex reveals a mosaic binding interface. Cell. 1999 Apr 16;97(2):271-81. [Article]
  10. Redfield C, Smith LJ, Boyd J, Lawrence GM, Edwards RG, Smith RA, Dobson CM: Secondary structure and topology of human interleukin 4 in solution. Biochemistry. 1991 Nov 19;30(46):11029-35. [Article]
  11. Smith LJ, Redfield C, Boyd J, Lawrence GM, Edwards RG, Smith RA, Dobson CM: Human interleukin 4. The solution structure of a four-helix bundle protein. J Mol Biol. 1992 Apr 20;224(4):899-904. [Article]
  12. Powers R, Garrett DS, March CJ, Frieden EA, Gronenborn AM, Clore GM: 1H, 15N, 13C, and 13CO assignments of human interleukin-4 using three-dimensional double- and triple-resonance heteronuclear magnetic resonance spectroscopy. Biochemistry. 1992 May 5;31(17):4334-46. [Article]
  13. Garrett DS, Powers R, March CJ, Frieden EA, Clore GM, Gronenborn AM: Determination of the secondary structure and folding topology of human interleukin-4 using three-dimensional heteronuclear magnetic resonance spectroscopy. Biochemistry. 1992 May 5;31(17):4347-53. [Article]
  14. Powers R, Garrett DS, March CJ, Frieden EA, Gronenborn AM, Clore GM: Three-dimensional solution structure of human interleukin-4 by multidimensional heteronuclear magnetic resonance spectroscopy. Science. 1992 Jun 19;256(5064):1673-7. [Article]
  15. Curtis BM, Presnell SR, Srinivasan S, Sassenfeld H, Klinke R, Jeffery E, Cosman D, March CJ, Cohen FE: Experimental and theoretical studies of the three-dimensional structure of human interleukin-4. Proteins. 1991;11(2):111-9. [Article]
  16. Muller T, Dieckmann T, Sebald W, Oschkinat H: Aspects of receptor binding and signalling of interleukin-4 investigated by site-directed mutagenesis and NMR spectroscopy. J Mol Biol. 1994 Apr 8;237(4):423-36. [Article]
  17. Smith LJ, Redfield C, Smith RA, Dobson CM, Clore GM, Gronenborn AM, Walter MR, Naganbushan TL, Wlodawer A: Comparison of four independently determined structures of human recombinant interleukin-4. Nat Struct Biol. 1994 May;1(5):301-10. [Article]
  18. Zee RY, Cook NR, Cheng S, Reynolds R, Erlich HA, Lindpaintner K, Ridker PM: Polymorphism in the P-selectin and interleukin-4 genes as determinants of stroke: a population-based, prospective genetic analysis. Hum Mol Genet. 2004 Feb 15;13(4):389-96. Epub 2003 Dec 17. [Article]

Drug Relations

Drug Relations
DrugBank IDNameDrug groupPharmacological action?ActionsDetails
DB06560PascolizumabinvestigationalunknownDetails
DB12159Dupilumabapproved, investigationalyesinhibitorantibodyDetails
DB16318RomilkimabinvestigationalunknowninhibitorDetails