Cytochrome P450 2A1

Details

Name
Cytochrome P450 2A1
Synonyms
  • 1.14.14.1
  • Cyp2a-1
  • CYPIIA1
  • Cytochrome P450-UT-F
  • Steroid hormones 7-alpha-hydroxylase
  • Testosterone 7-alpha-hydroxylase
Gene Name
Cyp2a1
Organism
Rat
Amino acid sequence
>lcl|BSEQ0021410|Cytochrome P450 2A1
MLDTGLLLVVILASLSVMLLVSLWQQKIRGRLPPGPTPLPFIGNYLQLNTKDVYSSITQL
SERYGPVFTIHLGPRRVVVLYGYDAVKEALVDQAEEFSGRGEQATYNTLFKGYGVAFSSG
ERAKQLRRLSIATLRDFGVGKRGVEERILEEAGYLIKMLQGTCGAPIDPTIYLSKTVSNV
ISSIVFGERFDYEDTEFLSLLQMMGQMNRFAASPTGQLYDMFHSVMKYLPGPQQQIIKVT
QKLEDFMIEKVRQNHSTLDPNSPRNFIDSFLIRMQEEKNGNSEFHMKNLVMTTLSLFFAG
SETVSSTLRYGFLLLMKHPDVEAKVHEEIEQVIGRNRQPQYEDHMKMPYTQAVINEIQRF
SNLAPLGIPRRIIKNTTFRGFFLPKGTDVFPILGSLMTDPKFFPSPKDFDPQNFLDDKGQ
LKKNAAFLPFSTGKRFCLGDGLAKMELFLLLTTILQNFRFKFPMKLEDINESPKPLGFTR
IIPKYTMSFMPI
Number of residues
492
Molecular Weight
55994.635
Theoretical pI
Not Available
GO Classification
Functions
arachidonic acid epoxygenase activity / aromatase activity / heme binding / iron ion binding / oxidoreductase activity / oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen / oxygen binding / steroid hydroxylase activity
Processes
coumarin metabolic process / epoxygenase P450 pathway / exogenous drug catabolic process / oxidation-reduction process / xenobiotic metabolic process
Components
cytoplasm / endoplasmic reticulum membrane / intracellular membrane-bounded organelle
General Function
Steroid hydroxylase activity
Specific Function
Highly active in the 7-alpha-hydroxylation of testosterone, progesterone and androstenedione.
Pfam Domain Function
Transmembrane Regions
Not Available
Cellular Location
Endoplasmic reticulum membrane
Gene sequence
>lcl|BSEQ0021411|Cytochrome P450 2A1 (Cyp2a1)
ATGCTGGACACAGGACTGCTTCTGGTGGTCATATTGGCCTCCCTGAGCGTCATGCTCTTG
GTGTCCCTCTGGCAGCAGAAAATCAGGGGGAGATTGCCTCCAGGACCCACTCCTTTGCCT
TTCATTGGAAATTATCTGCAGCTGAATACAAAAGACGTATACAGTTCCATCACACAGCTC
AGTGAGCGCTATGGTCCTGTGTTCACCATCCACCTTGGGCCTCGCCGGGTTGTGGTGCTT
TATGGATACGATGCAGTCAAAGAGGCTTTGGTGGACCAAGCTGAGGAGTTCAGTGGACGA
GGCGAACAGGCTACCTACAATACACTCTTCAAAGGCTATGGCGTGGCATTCAGCAGTGGG
GAGCGGGCAAAACAACTCAGGCGCCTCTCTATAGCCACATTGAGAGATTTTGGTGTGGGC
AAGCGTGGTGTAGAGGAGCGTATCCTGGAGGAGGCAGGCTATTTGATCAAGATGTTGCAG
GGCACTTGTGGAGCCCCCATTGACCCCACCATCTACCTGAGCAAAACAGTCTCCAATGTT
ATTAGCTCCATTGTCTTCGGGGAACGCTTCGACTATGAGGACACGGAGTTCCTGTCACTG
CTGCAGATGATGGGTCAAATGAACAGATTTGCAGCTTCACCCACAGGGCAGCTCTATGAC
ATGTTCCATTCAGTGATGAAGTACCTGCCTGGACCACAGCAACAGATCATCAAGGTTACT
CAGAAACTGGAAGACTTCATGATAGAGAAAGTGAGGCAGAACCATAGTACCCTGGACCCC
AATTCCCCAAGGAACTTCATTGACTCCTTTCTCATCCGCATGCAAGAGGAGAAAAATGGC
AATTCAGAGTTCCACATGAAGAACCTAGTGATGACAACACTAAGCCTCTTCTTTGCTGGG
TCTGAGACAGTCAGCTCCACACTACGCTACGGCTTCCTTCTACTCATGAAGCATCCAGAT
GTGGAGGCCAAGGTCCATGAGGAAATTGAGCAGGTGATCGGCAGGAACCGACAGCCTCAG
TATGAGGACCACATGAAGATGCCCTACACCCAGGCTGTGATCAATGAGATCCAAAGATTT
TCTAACTTGGCTCCCTTGGGCATTCCTCGAAGGATTATCAAGAACACAACCTTCCGTGGC
TTCTTCCTCCCCAAGGCCACCGATGTGTTCCCTATATTAGGTTCTCTGATGACAGACCCA
AAGTTCTTCCCTAGCCCCAAAGACTTCGACCCCCAGAACTTCCTGGATGACAAGGGACAG
TTGAAGAAAAATGCTGCTTTCCTCCCTTTCTCCACTGGGAAGCGATTCTGCTTGGGAGAT
GGCCTGGCTAAGATGGAGCTCTTCCTGCTGCTCACCACTATTTTACAGAACTTCCGTTTC
AAGTTCCCAATGAAACTAGAAGACATCAACGAGTCCCCCAAACCCTTGGGGTTTACCAGG
ATCATACCAAAGTACACCATGAGCTTCATGCCCATCTGA
Chromosome Location
1
Locus
Not Available
External Identifiers
ResourceLink
UniProtKB IDP11711
UniProtKB Entry NameCP2A1_RAT
General References
  1. Nagata K, Matsunaga T, Gillette J, Gelboin HV, Gonzalez FJ: Rat testosterone 7 alpha-hydroxylase. Isolation, sequence, and expression of cDNA and its developmental regulation and induction by 3-methylcholanthrene. J Biol Chem. 1987 Feb 25;262(6):2787-93. [Article]
  2. Matsunaga T, Nomoto M, Kozak CA, Gonzalez FJ: Structure and in vitro transcription of the rat CYP2A1 and CYP2A2 genes and regional localization of the CYP2A gene subfamily on mouse chromosome 7. Biochemistry. 1990 Feb 6;29(5):1329-41. [Article]
  3. Gerhard DS, Wagner L, Feingold EA, Shenmen CM, Grouse LH, Schuler G, Klein SL, Old S, Rasooly R, Good P, Guyer M, Peck AM, Derge JG, Lipman D, Collins FS, Jang W, Sherry S, Feolo M, Misquitta L, Lee E, Rotmistrovsky K, Greenhut SF, Schaefer CF, Buetow K, Bonner TI, Haussler D, Kent J, Kiekhaus M, Furey T, Brent M, Prange C, Schreiber K, Shapiro N, Bhat NK, Hopkins RF, Hsie F, Driscoll T, Soares MB, Casavant TL, Scheetz TE, Brown-stein MJ, Usdin TB, Toshiyuki S, Carninci P, Piao Y, Dudekula DB, Ko MS, Kawakami K, Suzuki Y, Sugano S, Gruber CE, Smith MR, Simmons B, Moore T, Waterman R, Johnson SL, Ruan Y, Wei CL, Mathavan S, Gunaratne PH, Wu J, Garcia AM, Hulyk SW, Fuh E, Yuan Y, Sneed A, Kowis C, Hodgson A, Muzny DM, McPherson J, Gibbs RA, Fahey J, Helton E, Ketteman M, Madan A, Rodrigues S, Sanchez A, Whiting M, Madari A, Young AC, Wetherby KD, Granite SJ, Kwong PN, Brinkley CP, Pearson RL, Bouffard GG, Blakesly RW, Green ED, Dickson MC, Rodriguez AC, Grimwood J, Schmutz J, Myers RM, Butterfield YS, Griffith M, Griffith OL, Krzywinski MI, Liao N, Morin R, Palmquist D, Petrescu AS, Skalska U, Smailus DE, Stott JM, Schnerch A, Schein JE, Jones SJ, Holt RA, Baross A, Marra MA, Clifton S, Makowski KA, Bosak S, Malek J: The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Genome Res. 2004 Oct;14(10B):2121-7. [Article]
  4. Arlotto MP, Greenway DJ, Parkinson A: Purification of two isozymes of rat liver microsomal cytochrome P450 with testosterone 7 alpha-hydroxylase activity. Arch Biochem Biophys. 1989 May 1;270(2):441-57. [Article]
  5. Imaoka S, Kamataki T, Funae Y: Purification and characterization of six cytochromes P-450 from hepatic microsomes of immature female rats. J Biochem. 1987 Oct;102(4):843-51. [Article]
  6. Lundby A, Secher A, Lage K, Nordsborg NB, Dmytriyev A, Lundby C, Olsen JV: Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues. Nat Commun. 2012 Jun 6;3:876. doi: 10.1038/ncomms1871. [Article]

Drug Relations

Drug Relations
DrugBank IDNameDrug groupPharmacological action?ActionsDetails
DB08834Tauroursodeoxycholic acidexperimental, investigationalunknowninducerDetails
DB13746Bioallethrinapproved, experimentalnosubstrateDetails