Tail fiber protein
Details
- Name
- Tail fiber protein
- Synonyms
- 3.2.1.-
- Endo-1,3-alpha-L-rhamnosidase
- Endorhamnosidase
- Gene product 9
- gp9
- Tail spike protein
- TSP
- Gene Name
- 9
- Organism
- Enterobacteria phage P22
- Amino acid sequence
>lcl|BSEQ0010902|Tail fiber protein MTDITANVVVSNPRPIFTESRSFKAVANGKIYIGQIDTDPVNPANQIPVYIENEDGSHVQ ITQPLIINAAGKIVYNGQLVKIVTVQGHSMAIYDANGSQVDYIANVLKYDPDQYSIEADK KFKYSVKLSDYPTLQDAASAAVDGLLIDRDYNFYGGETVDFGGKVLTIECKAKFIGDGNL IFTKLGKGSRIAGVFMESTTTPWVIKPWTDDNQWLTDAAAVVATLKQSKTDGYQPTVSDY VKFPGIETLLPPNAKGQNITSTLEIRECIGVEVHRASGLMAGFLFRGCHFCKMVDANNPS GGKDGIITFENLSGDWGKGNYVIGGRTSYGSVSSAQFLRNNGGFERDGGVIGFTSYRAGE SGVKTWQGTVGSTTSRNYNLQFRDSVVIYPVWDGFDLGADTDMNPELDRPGDYPITQYPL HQLPLNHLIDNLLVRGALGVGFGMDGKGMYVSNITVEDCAGSGAYLLTHESVFTNIAIID TNTKDFQANQIYISGACRVNGLRLIGIRSTDGQGLTIDAPNSTVSGITGMVDPSRINVAN LAEEGLGNIRANSFGYDSAAIKLRIHKLSKTLDSGALYSHINGGAGSGSAYTQLTAISGS TPDAVSLKVNHKDCRGAEIPFVPDIASDDFIKDSSCFLPYWENNSTSLKALVKKPNGELV RLTLATL
- Number of residues
- 667
- Molecular Weight
- 71856.28
- Theoretical pI
- 5.25
- GO Classification
- Functionshydrolase activity, acting on glycosyl bondsProcessespathogenesis / viral entry into host cell / virion attachment to host cellComponentsvirion
- General Function
- Hydrolase activity, acting on glycosyl bonds
- Specific Function
- Structural component of the short non-contractile tail. The tail comprises six fibers that mediate primary attachment to the host cell lipopolysaccharides (LPS) and display endorhamnosidase enzymatic activity, hydrolyzing the alpha-1,3-O-glycosidic linkage between rhamnose and galactose of the O-antigen polysaccharide. Digestion of the LPS brings the capsid near the cell outer membrane.
- Pfam Domain Function
- Transmembrane Regions
- Not Available
- Cellular Location
- Virion
- Gene sequence
>lcl|BSEQ0010903|Tail fiber protein (9) ATGACAGACATCACTGCAAACGTAGTTGTTTCTAACCCTCGTCCAATCTTCACTGAATCC CGTTCGTTTAAAGCTGTTGCTAATGGGAAAATTTACATTGGTCAGATTGATACCGATCCG GTTAATCCTGCCAATCAGATACCCGTATACATTGAAAATGAGGATGGCTCTCACGTCCAG ATTACTCAGCCGCTAATTATCAACGCAGCCGGTAAAATCGTATACAACGGCCAACTGGTG AAAATTGTCACCGTTCAGGGTCATAGCATGGCTATCTATGATGCCAATGGTTCTCAGGTT GACTATATTGCTAACGTATTGAAGTACGATCCAGATCAATATTCAATAGAAGCTGATAAA AAATTTAAGTATTCAGTAAAATTATCAGATTATCCAACATTGCAGGATGCAGCATCTGCT GCGGTTGATGGCCTTCTTATCGATCGAGATTATAATTTTTATGGTGGAGAGACAGTTGAT TTTGGCGGAAAGGTTCTGACTATAGAATGTAAAGCTAAATTTATAGGAGATGGAAATCTT ATTTTTACGAAATTAGGCAAAGGTTCCCGCATTGCCGGGGTTTTTATGGAAAGCACTACA ACACCATGGGTTATCAAGCCTTGGACGGATGACAATCAGTGGCTAACGGATGCCGCAGCG GTCGTTGCCACTTTAAAACAATCTAAAACTGATGGGTATCAGCCAACCGTAAGCGATTAC GTTAAATTCCCAGGAATAGAAACGTTACTCCCACCTAATGCAAAAGGGCAAAACATAACG TCTACGTTAGAAATTAGAGAATGTATAGGGGTCGAAGTTCATCGGGCTAGCGGTCTAATG GCTGGTTTTTTGTTTAGAGGGTGTCACTTCTGCAAGATGGTAGACGCCAATAATCCAAGC GGAGGTAAAGATGGCATTATAACCTTCGAAAACCTTAGCGGCGATTGGGGGAAGGGTAAC TATGTCATTGGCGGACGAACCAGCTATGGGTCAGTAAGTAGCGCCCAGTTTTTACGTAAT AATGGTGGCTTTGAACGTGATGGTGGAGTTATTGGGTTTACTTCATATCGCGCTGGGGAG AGTGGCGTTAAAACTTGGCAAGGTACTGTGGGCTCGACAACCTCTCGCAACTATAATCTG CAATTCCGCGACTCGGTCGTTATTTACCCCGTATGGGACGGATTCGATTTAGGTGCTGAC ACTGACATGAATCCGGAGTTGGACAGGCCAGGGGACTACCCTATAACCCAATACCCACTG CATCAGTTACCCCTAAATCACCTGATTGATAATCTTCTGGTTCGCGGGGCGTTAGGTGTA GGTTTTGGTATGGATGGTAAGGGCATGTATGTGTCTAATATTACCGTAGAAGATTGCGCT GGGTCTGGCGCGTACCTACTCACCCACGAATCAGTATTTACCAATATAGCCATAATTGAC ACCAATACTAAGGATTTCCAGGCGAATCAGATTTATATATCTGGGGCTTGCCGTGTGAAC GGTTTACGTTTAATTGGGATCCGCTCAACCGATGGGCAGGGTCTAACCATAGACGCCCCT AACTCTACCGTAAGCGGTATAACCGGGATGGTAGACCCCTCTAGAATTAATGTTGCTAAT TTGGCAGAAGAAGGGTTAGGTAATATCCGCGCTAATAGTTTCGGCTATGATAGCGCAGCG ATTAAACTGCGGATTCATAAGTTATCAAAGACATTAGATAGCGGAGCATTGTACTCCCAC ATTAACGGGGGGGCCGGTTCTGGCTCAGCGTATACTCAACTTACTGCTATTTCAGGTAGC ACACCTGACGCTGTATCATTAAAAGTTAACCACAAAGATTGCAGGGGGGCAGAGATACCA TTTGTTCCTGACATCGCGTCAGATGATTTTATAAAGGATTCCTCATGTTTTTTGCCATAT TGGGAAAATAATTCTACTTCTTTAAAGGCTTTAGTGAAAAAACCCAATGGAGAATTAGTT AGATTAACCTTGGCAACACTTTAG
- Chromosome Location
- Not Available
- Locus
- Not Available
- External Identifiers
Resource Link UniProtKB ID P12528 UniProtKB Entry Name FIBER_BPP22 GenBank Gene ID J02473 - General References
- Sauer RT, Krovatin W, Poteete AR, Berget PB: Phage P22 tail protein: gene and amino acid sequence. Biochemistry. 1982 Nov 9;21(23):5811-5. [Article]
- Vander Byl C, Kropinski AM: Sequence of the genome of Salmonella bacteriophage P22. J Bacteriol. 2000 Nov;182(22):6472-81. [Article]
- Pedulla ML, Ford ME, Karthikeyan T, Houtz JM, Hendrix RW, Hatfull GF, Poteete AR, Gilcrease EB, Winn-Stapley DA, Casjens SR: Corrected sequence of the bacteriophage p22 genome. J Bacteriol. 2003 Feb;185(4):1475-7. [Article]
- Weigele PR, Scanlon E, King J: Homotrimeric, beta-stranded viral adhesins and tail proteins. J Bacteriol. 2003 Jul;185(14):4022-30. [Article]
- Andres D, Hanke C, Baxa U, Seul A, Barbirz S, Seckler R: Tailspike interactions with lipopolysaccharide effect DNA ejection from phage P22 particles in vitro. J Biol Chem. 2010 Nov 19;285(47):36768-75. doi: 10.1074/jbc.M110.169003. Epub 2010 Sep 3. [Article]
- Steinbacher S, Seckler R, Miller S, Steipe B, Huber R, Reinemer P: Crystal structure of P22 tailspike protein: interdigitated subunits in a thermostable trimer. Science. 1994 Jul 15;265(5170):383-6. [Article]
- Steinbacher S, Baxa U, Miller S, Weintraub A, Seckler R, Huber R: Crystal structure of phage P22 tailspike protein complexed with Salmonella sp. O-antigen receptors. Proc Natl Acad Sci U S A. 1996 Oct 1;93(20):10584-8. [Article]
- Steinbacher S, Miller S, Baxa U, Budisa N, Weintraub A, Seckler R, Huber R: Phage P22 tailspike protein: crystal structure of the head-binding domain at 2.3 A, fully refined structure of the endorhamnosidase at 1.56 A resolution, and the molecular basis of O-antigen recognition and cleavage. J Mol Biol. 1997 Apr 11;267(4):865-80. [Article]
- Baxa U, Steinbacher S, Weintraub A, Huber R, Seckler R: Mutations improving the folding of phage P22 tailspike protein affect its receptor binding activity. J Mol Biol. 1999 Oct 29;293(3):693-701. [Article]
- Schuler B, Furst F, Osterroth F, Steinbacher S, Huber R, Seckler R: Plasticity and steric strain in a parallel beta-helix: rational mutations in the P22 tailspike protein. Proteins. 2000 Apr 1;39(1):89-101. [Article]