Desvancosaminyl-vancomycin vancosaminetransferase

Details

Name
Desvancosaminyl-vancomycin vancosaminetransferase
Synonyms
  • 2.4.1.322
  • Desvancosamine-vancomycin TDP-vancosaminyltransferase
  • Glycosyltransferase GtfD
Gene Name
gtfD
UniProtKB Entry
Q9AFC7Swiss-Prot
Organism
Amycolatopsis orientalis
NCBI Taxonomy ID
31958
Amino acid sequence
>lcl|BSEQ0012323|Desvancosaminyl-vancomycin vancosaminetransferase
MRVLLSVCGTRGDVEIGVALADRLKALGVQTRMCAPPAAEERLAEVGVPHVPVGLPQHMM
LQEGMPPPPPEEEQRLAAMTVEMQFDAVPGAAEGCAAVVAVGDLAAATGVRSVAEKLGLP
FFYSVPSPVYLASPHLPPAYDEPTTPGVTDIRVLWEERAARFADRYGPTLNRRRAEIGLP
PVEDVFGYGHGERPCWPADPVLAPLQPDVDAVQTGAWLLSDERPLPPELEAFLAAGSPPV
HIGFGSSSGRGIADAAKVAVEAIRAQGRRVILSRGWTELVLPDDRDDCFAIDEVNFQALF
RRVAAVIHHGSAGTEHVATRAGVPQLVIPRNTDQPYFAGRVAALGIGVAHDGPTPTFESL
SAALTTVLAPETRARAEAVAGMVLTDGAAAAADLVLAAVGREKPAVPA
Number of residues
408
Molecular Weight
42880.545
Theoretical pI
4.8
GO Classification
Functions
transferase activity, transferring hexosyl groups
Processes
lipid glycosylation / vancomycin biosynthetic process
General Function
Catalyzes the attachment of L-vancosamine to a monoglucosylated heptapeptide intermediate during the final stage of glycopeptide antibiotic vancomycin biosynthesis.
Specific Function
enzyme binding
Pfam Domain Function
Signal Regions
Not Available
Transmembrane Regions
Not Available
Cellular Location
Not Available
Gene sequence
>lcl|BSEQ0006478|1227 bp
ATGCGTGTGTTGTTGTCGGTGTGCGGAACCCGCGGGGACGTCGAAATCGGGGTGGCGCTG
GCGGACCGATTGAAGGCGCTCGGCGTCCAGACACGGATGTGCGCGCCGCCCGCCGCCGAG
GAGCGGCTGGCGGAGGTCGGGGTGCCGCATGTGCCGGTCGGCCTGCCGCAGCACATGATG
TTGCAGGAGGGCATGCCGCCGCCACCGCCCGAAGAGGAACAGCGGCTGGCGGCCATGACG
GTCGAGATGCAGTTCGACGCCGTCCCCGGGGCCGCCGAAGGGTGTGCCGCGGTGGTGGCG
GTCGGCGATCTGGCCGCCGCGACCGGTGTCCGGTCGGTGGCCGAGAAACTGGGCCTGCCC
TTCTTCTATTCCGTCCCCAGTCCGGTCTATCTGGCGTCCCCGCACCTTCCGCCGGCTTAC
GACGAGCCGACCACGCCGGGCGTGACCGACATCCGGGTGCTGTGGGAGGAGCGGGCCGCC
CGGTTCGCCGACCGGTACGGGCCCACGCTCAACCGTCGCCGGGCCGAGATCGGCCTGCCG
CCGGTGGAGGACGTCTTCGGCTACGGCCACGGTGAGCGGCCCTGCTGGCCGGCGGACCCG
GTACTGGCGCCCCTGCAGCCGGACGTCGACGCGGTGCAGACCGGTGCGTGGCTCCTGTCC
GACGAGCGGCCGCTTCCCCCGGAGCTGGAAGCGTTCCTGGCCGCCGGGTCACCGCCGGTG
CACATCGGTTTCGGCAGCTCGTCCGGCCGCGGGATCGCCGACGCCGCGAAGGTGGCCGTC
GAGGCGATCCGGGCCCAAGGCCGGCGGGTGATCCTCTCGCGGGGGTGGACCGAGCTGGTC
CTGCCCGACGACCGTGACGACTGTTTCGCCATCGACGAGGTGAACTTCCAGGCGCTGTTC
CGCCGGGTGGCCGCCGTCATCCACCACGGCAGCGCGGGTACGGAGCACGTGGCCACGCGG
GCCGGCGTCCCCCAGCTCGTGATCCCCCGGAACACGGACCAGCCGTACTTCGCCGGGCGG
GTGGCCGCGCTGGGGATCGGTGTGGCGCATGACGGCCCGACGCCGACCTTCGAGTCCCTT
TCGGCCGCGCTCACCACGGTGCTCGCGCCGGAGACGCGGGCGCGGGCGGAGGCCGTGGCG
GGCATGGTCCTCACCGACGGGGCGGCGGCGGCCGCGGATCTGGTGCTCGCCGCGGTCGGC
CGGGAGAAGCCCGCCGTTCCCGCGTGA
Chromosome Location
Not Available
Locus
Not Available
External Identifiers
ResourceLink
UniProtKB IDQ9AFC7
UniProtKB Entry NameGTFD_AMYOR
GenBank Gene IDAF351621
PDB ID(s)1RRV
General References
  1. Losey HC, Peczuh MW, Chen Z, Eggert US, Dong SD, Pelczer I, Kahne D, Walsh CT: Tandem action of glycosyltransferases in the maturation of vancomycin and teicoplanin aglycones: novel glycopeptides. Biochemistry. 2001 Apr 17;40(15):4745-55. [Article]
  2. Mulichak AM, Lu W, Losey HC, Walsh CT, Garavito RM: Crystal structure of vancosaminyltransferase GtfD from the vancomycin biosynthetic pathway: interactions with acceptor and nucleotide ligands. Biochemistry. 2004 May 11;43(18):5170-80. [Article]

Associated Data

Bio-Entities
Bio-EntityType
Desvancosaminyl-vancomycin vancosaminetransferase (Amycolatopsis orientalis)protein
primary
Drug Relations
DrugDrug groupPharmacological action?TypeActionsDetails
Desvancosaminyl vancomycinexperimentalunknowntargetDetails