Cloning of the pyridoxine 5'-phosphate phosphatase gene (pdxP) and vitamin B6 production in pdxP recombinant Sinorhizobium meliloti.
Article Details
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Nagahashi Y, Tazoe M, Hoshino T
Cloning of the pyridoxine 5'-phosphate phosphatase gene (pdxP) and vitamin B6 production in pdxP recombinant Sinorhizobium meliloti.
Biosci Biotechnol Biochem. 2008 Feb;72(2):421-7. Epub 2008 Feb 7.
- PubMed ID
- 18256491 [ View in PubMed]
- Abstract
A novel gene (pdxP) encoding a pyridoxine 5'-phosphate (PNP) phosphatase involved in the last step of pyridoxine biosynthesis was cloned from Sinorhizobium meliloti IFO 14782 on the basis of the peptide sequences of the natural enzyme. The pdxP gene is an open reading frame (708 bp) encoding 235 amino acid residues with a calculated molecular weight of 26,466. From its deduced amino acid sequence, it was predicted that the enzyme belongs to the haloacid dehalogenase superfamily. Transformants of Escherichia coli and S. meliloti by pdxP gene expression plasmids showed stimulated PNP phosphatase activities. When pdxP was overexpressed together with the PNP synthase gene (pdxJ) in S. meliloti, the recombinant strain produced 149 mg/l of pyridoxine, 46% and 16% higher than the host strain and the pdxJ recombinant of S. meliloti respectively.
DrugBank Data that Cites this Article
- Drug Targets
Drug Target Kind Organism Pharmacological Action Actions Pyridoxine Pyridoxal kinase Protein Humans YesLigandDetails - Drug Enzymes
Drug Enzyme Kind Organism Pharmacological Action Actions Pyridoxine phosphate Pyridoxine 5'-phosphate phosphatase Protein Ensifer meliloti UnknownSubstrateDetails - Polypeptides
Name UniProt ID Pyridoxine 5'-phosphate phosphatase A7BK78 Details