rRNA Binding Sites and the Molecular Mechanism of Action of the Tetracyclines.

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Chukwudi CU

rRNA Binding Sites and the Molecular Mechanism of Action of the Tetracyclines.

Antimicrob Agents Chemother. 2016 Jul 22;60(8):4433-41. doi: 10.1128/AAC.00594-16. Print 2016 Aug.

PubMed ID

27246781 [ View in PubMed

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Abstract

The tetracycline antibiotics are known to be effective in the treatment of both infectious and noninfectious disease conditions. The 16S rRNA binding mechanism currently held for the antibacterial action of the tetracyclines does not explain their activity against viruses, protozoa that lack mitochondria, and noninfectious conditions. Also, the mechanism by which the tetracyclines selectively inhibit microbial protein synthesis against host eukaryotic protein synthesis despite conservation of ribosome structure and functions is still questionable. Many studies have investigated the binding of the tetracyclines to the 16S rRNA using the small ribosomal subunit of different bacterial species, but there seems to be no agreement between various reports on the exact binding site on the 16S rRNA. The wide range of activity of the tetracyclines against a broad spectrum of bacterial pathogens, viruses, protozoa, and helminths, as well as noninfectious conditions, indicates a more generalized effect on RNA. In the light of recent evidence that the tetracyclines bind to various synthetic double-stranded RNAs (dsRNAs) of random base sequences, suggesting that the double-stranded structures may play a more important role in the binding of the tetracyclines to RNA than the specific base pairs, as earlier speculated, it is imperative to consider possible alternative binding modes or sites that could help explain the mechanisms of action of the tetracyclines against various pathogens and disease conditions.

DrugBank Data that Cites this Article

Drugs

Lymecycline

Drug Targets

Drug	Target	Kind	Organism	Pharmacological Action	Actions
Lymecycline	30S ribosomal protein S4	Protein	Escherichia coli (strain K12)	Yes	Inhibitor	Details
Minocycline	16S ribosomal RNA	Nucleotide	Enteric bacteria and other eubacteria	Unknown	Inhibitor	Details