Identification of protein arginine methyltransferase 2 as a coactivator for estrogen receptor alpha.

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Qi C, Chang J, Zhu Y, Yeldandi AV, Rao SM, Zhu YJ

Identification of protein arginine methyltransferase 2 as a coactivator for estrogen receptor alpha.

J Biol Chem. 2002 Aug 9;277(32):28624-30. Epub 2002 May 30.

PubMed ID
12039952 [ View in PubMed
]
Abstract

In an attempt to isolate cofactors capable of influencing estrogen receptor alpha (ERalpha) transcriptional activity, we used yeast two-hybrid screening and identified protein arginine methyltransferase 2 (PRMT2) as a new ERalpha-binding protein. PRMT2 interacted directly with three ERalpha regions including AF-1, DNA binding domain, and hormone binding domain in a ligand-independent fashion. The ERalpha-interacting region on PRMT2 has been mapped to a region encompassing amino acids 133-275. PRMT2 also binds to ERbeta, PR, TRbeta, RARalpha, PPARgamma, and RXRalpha in a ligand-independent manner. PRMT2 enhanced both ERalpha AF-1 and AF-2 transcriptional activity, and the potential methyltransferase activity of PRMT2 appeared pivotal for its coactivator function. In addition, PRMT2 enhanced PR, PPARgamma, and RARalpha-mediated transactivation. Although PRMT2 was found to interact with two other coactivators, the steroid receptor coactivator-1 (SRC-1) and the peroxisome proliferator-activated receptor-interacting protein (PRIP), no synergistic enhancement of ERalpha transcriptional activity was observed when PRMT2 was coexpressed with either PRIP or SRC-1. In this respect PRMT2 differs from coactivators PRMT1 and CARM1 (coactivator-associated arginine methyltransferase). These results suggest that PRMT2 is a novel ERalpha coactivator.

DrugBank Data that Cites this Article

Polypeptides
NameUniProt ID
Estrogen receptorP03372Details
Peroxisome proliferator-activated receptor gammaP37231Details
Thyroid hormone receptor betaP10828Details
Retinoic acid receptor RXR-alphaP19793Details
Progesterone receptorP06401Details
Retinoic acid receptor alphaP10276Details
Estrogen receptor betaQ92731Details
Nuclear receptor coactivator 1Q15788Details