Global profiling of co- and post-translationally N-myristoylated proteomes in human cells.

Article Details


Thinon E, Serwa RA, Broncel M, Brannigan JA, Brassat U, Wright MH, Heal WP, Wilkinson AJ, Mann DJ, Tate EW

Global profiling of co- and post-translationally N-myristoylated proteomes in human cells.

Nat Commun. 2014 Sep 26;5:4919. doi: 10.1038/ncomms5919.

PubMed ID
25255805 [ View in PubMed

Protein N-myristoylation is a ubiquitous co- and post-translational modification that has been implicated in the development and progression of a range of human diseases. Here, we report the global N-myristoylated proteome in human cells determined using quantitative chemical proteomics combined with potent and specific human N-myristoyltransferase (NMT) inhibition. Global quantification of N-myristoylation during normal growth or apoptosis allowed the identification of >100 N-myristoylated proteins, >95% of which are identified for the first time at endogenous levels. Furthermore, quantitative dose response for inhibition of N-myristoylation is determined for >70 substrates simultaneously across the proteome. Small-molecule inhibition through a conserved substrate-binding pocket is also demonstrated by solving the crystal structures of inhibitor-bound NMT1 and NMT2. The presented data substantially expand the known repertoire of co- and post-translational N-myristoylation in addition to validating tools for the pharmacological inhibition of NMT in living cells.

DrugBank Data that Cites this Article

NameUniProt ID
NADH-cytochrome b5 reductase 3P00387Details
Calcineurin subunit B type 1P63098Details
Golgi-associated plant pathogenesis-related protein 1Q9H4G4Details
ADP-ribosylation factor 4P18085Details
cAMP-dependent protein kinase catalytic subunit alphaP17612Details
Glycylpeptide N-tetradecanoyltransferase 2O60551Details
ADP-ribosylation factor 1P84077Details
60S ribosomal protein L15P61313Details
ADP-ribosylation factor 6P62330Details
Tyrosine-protein kinase LynP07948Details
Glycylpeptide N-tetradecanoyltransferase 1P30419Details
cAMP-dependent protein kinase catalytic subunit betaP22694Details
Guanine nucleotide-binding protein G(i) subunit alpha-1P63096Details
ADP-ribosylation factor-like protein 1P40616Details
Neuronal calcium sensor 1P62166Details
Calcineurin B homologous protein 1Q99653Details
40S ribosomal protein S8P62241Details