Cloning and functional analysis of cDNAs with open reading frames for 300 previously undefined genes expressed in CD34+ hematopoietic stem/progenitor cells.

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Zhang QH, Ye M, Wu XY, Ren SX, Zhao M, Zhao CJ, Fu G, Shen Y, Fan HY, Lu G, Zhong M, Xu XR, Han ZG, Zhang JW, Tao J, Huang QH, Zhou J, Hu GX, Gu J, Chen SJ, Chen Z

Cloning and functional analysis of cDNAs with open reading frames for 300 previously undefined genes expressed in CD34+ hematopoietic stem/progenitor cells.

Genome Res. 2000 Oct;10(10):1546-60.

PubMed ID
11042152 [ View in PubMed
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Abstract

Three hundred cDNAs containing putatively entire open reading frames (ORFs) for previously undefined genes were obtained from CD34+ hematopoietic stem/progenitor cells (HSPCs), based on EST cataloging, clone sequencing, in silico cloning, and rapid amplification of cDNA ends (RACE). The cDNA sizes ranged from 360 to 3496 bp and their ORFs coded for peptides of 58-752 amino acids. Public database search indicated that 225 cDNAs exhibited sequence similarities to genes identified across a variety of species. Homology analysis led to the recognition of 50 basic structural motifs/domains among these cDNAs. Genomic exon-intron organization could be established in 243 genes by integration of cDNA data with genome sequence information. Interestingly, a new gene named as HSPC070 on 3p was found to share a sequence of 105bp in 3' UTR with RAF gene in reversed transcription orientation. Chromosomal localizations were obtained using electronic mapping for 192 genes and with radiation hybrid (RH) for 38 genes. Macroarray technique was applied to screen the gene expression patterns in five hematopoietic cell lines (NB4, HL60, U937, K562, and Jurkat) and a number of genes with differential expression were found. The resource work has provided a wide range of information useful not only for expression genomics and annotation of genomic DNA sequence, but also for further research on the function of genes involved in hematopoietic development and differentiation.

DrugBank Data that Cites this Article

Polypeptides
NameUniProt ID
NADH dehydrogenase [ubiquinone] 1 subunit C2O95298Details
NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10O96000Details
Methionine-R-sulfoxide reductase B1Q9NZV6Details
NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6O95139Details
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7O95182Details
NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrialO95178Details
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2O43678Details
Phenylalanine--tRNA ligase beta subunitQ9NSD9Details
NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9Q9Y6M9Details
N(G),N(G)-dimethylarginine dimethylaminohydrolase 2O95865Details
NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrialO95169Details
5'-AMP-activated protein kinase catalytic subunit alpha-1Q13131Details
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3O95167Details
Glutathione S-transferase kappa 1Q9Y2Q3Details
m7GpppX diphosphataseQ96C86Details
Dual adapter for phosphotyrosine and 3-phosphotyrosine and 3-phosphoinositideQ9UN19Details
Glutamyl-tRNA(Gln) amidotransferase subunit B, mitochondrialO75879Details
60S ribosome subunit biogenesis protein NIP7 homologQ9Y221Details
60S ribosomal protein L26-like 1Q9UNX3Details
Cytochrome b-c1 complex subunit 9Q9UDW1Details
Proteasome subunit alpha type-7O14818Details
Trafficking protein particle complex subunit 4Q9Y296Details
Diphthine methyl ester synthaseQ9H2P9Details
tRNA-splicing ligase RtcB homologQ9Y3I0Details
GTP-binding nuclear protein RanP62826Details
Alpha-hemoglobin-stabilizing proteinQ9NZD4Details
ATP synthase subunit g, mitochondrialO75964Details