m7GpppX diphosphatase


m7GpppX diphosphatase
  • DCS-1
  • DCS1
  • Decapping scavenger enzyme
  • HINT-5
  • Hint-related 7meGMP-directed hydrolase
  • HINT5
  • Histidine triad nucleotide-binding protein 5
  • Histidine triad protein member 5
  • Scavenger mRNA-decapping enzyme DcpS
Gene Name
Amino acid sequence
>lcl|BSEQ0010846|m7GpppX diphosphatase
Number of residues
Molecular Weight
Theoretical pI
GO Classification
m7G(5')pppN diphosphatase activity / RNA 7-methylguanosine cap binding
cellular response to menadione / deadenylation-dependent decapping of nuclear-transcribed mRNA / exonucleolytic nuclear-transcribed mRNA catabolic process involved in deadenylation-dependent decay / gene expression / mRNA cis splicing, via spliceosome / negative regulation of programmed cell death / nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay
cytoplasm / cytosol / mitochondrion / nucleoplasm / nucleus
General Function
Rna 7-methylguanosine cap binding
Specific Function
Decapping scavenger enzyme that catalyzes the cleavage of a residual cap structure following the degradation of mRNAs by the 3'->5' exosome-mediated mRNA decay pathway. Hydrolyzes cap analog structures like 7-methylguanosine nucleoside triphosphate (m7GpppG) with up to 10 nucleotide substrates (small capped oligoribonucleotides) and specifically releases 5'-phosphorylated RNA fragments and 7-methylguanosine monophosphate (m7GMP). Cleaves cap analog structures like tri-methyl guanosine nucleoside triphosphate (m3(2,2,7)GpppG) with very poor efficiency. Does not hydrolyze unmethylated cap analog (GpppG) and shows no decapping activity on intact m7GpppG-capped mRNA molecules longer than 25 nucleotides. Does not hydrolyze 7-methylguanosine diphosphate (m7GDP) to m7GMP (PubMed:22985415). May also play a role in the 5'->3 mRNA decay pathway; m7GDP, the downstream product released by the 5'->3' mRNA mediated decapping activity, may be also converted by DCPS to m7GMP (PubMed:14523240). Binds to m7GpppG and strongly to m7GDP. Plays a role in first intron splicing of pre-mRNAs. Inhibits activation-induced cell death.
Pfam Domain Function
Transmembrane Regions
Not Available
Cellular Location
Gene sequence
>lcl|BSEQ0010847|m7GpppX diphosphatase (DCPS)
Chromosome Location
External Identifiers
UniProtKB IDQ96C86
UniProtKB Entry NameDCPS_HUMAN
GenBank Protein ID21654920
GenBank Gene IDAY040773
General References
  1. Liu H, Rodgers ND, Jiao X, Kiledjian M: The scavenger mRNA decapping enzyme DcpS is a member of the HIT family of pyrophosphatases. EMBO J. 2002 Sep 2;21(17):4699-708. [Article]
  2. Kwasnicka DA, Krakowiak A, Thacker C, Brenner C, Vincent SR: Coordinate expression of NADPH-dependent flavin reductase, Fre-1, and Hint-related 7meGMP-directed hydrolase, DCS-1. J Biol Chem. 2003 Oct 3;278(40):39051-8. Epub 2003 Jul 18. [Article]
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  5. Wang Z, Kiledjian M: Functional link between the mammalian exosome and mRNA decapping. Cell. 2001 Dec 14;107(6):751-62. [Article]
  6. van Dijk E, Le Hir H, Seraphin B: DcpS can act in the 5'-3' mRNA decay pathway in addition to the 3'-5' pathway. Proc Natl Acad Sci U S A. 2003 Oct 14;100(21):12081-6. Epub 2003 Oct 1. [Article]
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  8. Cohen LS, Mikhli C, Friedman C, Jankowska-Anyszka M, Stepinski J, Darzynkiewicz E, Davis RE: Nematode m7GpppG and m3(2,2,7)GpppG decapping: activities in Ascaris embryos and characterization of C. elegans scavenger DcpS. RNA. 2004 Oct;10(10):1609-24. [Article]
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  12. Mariller C, Hardiville S, Hoedt E, Benaissa M, Mazurier J, Pierce A: Proteomic approach to the identification of novel delta-lactoferrin target genes: Characterization of DcpS, an mRNA scavenger decapping enzyme. Biochimie. 2009 Jan;91(1):109-22. doi: 10.1016/j.biochi.2008.07.009. Epub 2008 Aug 7. [Article]
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  18. Bian Y, Song C, Cheng K, Dong M, Wang F, Huang J, Sun D, Wang L, Ye M, Zou H: An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome. J Proteomics. 2014 Jan 16;96:253-62. doi: 10.1016/j.jprot.2013.11.014. Epub 2013 Nov 22. [Article]
  19. Ng CK, Shboul M, Taverniti V, Bonnard C, Lee H, Eskin A, Nelson SF, Al-Raqad M, Altawalbeh S, Seraphin B, Reversade B: Loss of the scavenger mRNA decapping enzyme DCPS causes syndromic intellectual disability with neuromuscular defects. Hum Mol Genet. 2015 Jun 1;24(11):3163-71. doi: 10.1093/hmg/ddv067. Epub 2015 Feb 24. [Article]
  20. Ahmed I, Buchert R, Zhou M, Jiao X, Mittal K, Sheikh TI, Scheller U, Vasli N, Rafiq MA, Brohi MQ, Mikhailov A, Ayaz M, Bhatti A, Sticht H, Nasr T, Carter MT, Uebe S, Reis A, Ayub M, John P, Kiledjian M, Vincent JB, Jamra RA: Mutations in DCPS and EDC3 in autosomal recessive intellectual disability indicate a crucial role for mRNA decapping in neurodevelopment. Hum Mol Genet. 2015 Jun 1;24(11):3172-80. doi: 10.1093/hmg/ddv069. Epub 2015 Feb 20. [Article]
  21. Gu M, Fabrega C, Liu SW, Liu H, Kiledjian M, Lima CD: Insights into the structure, mechanism, and regulation of scavenger mRNA decapping activity. Mol Cell. 2004 Apr 9;14(1):67-80. [Article]
  22. Chen N, Walsh MA, Liu Y, Parker R, Song H: Crystal structures of human DcpS in ligand-free and m7GDP-bound forms suggest a dynamic mechanism for scavenger mRNA decapping. J Mol Biol. 2005 Apr 8;347(4):707-18. [Article]
  23. Singh J, Salcius M, Liu SW, Staker BL, Mishra R, Thurmond J, Michaud G, Mattoon DR, Printen J, Christensen J, Bjornsson JM, Pollok BA, Kiledjian M, Stewart L, Jarecki J, Gurney ME: DcpS as a therapeutic target for spinal muscular atrophy. ACS Chem Biol. 2008 Nov 21;3(11):711-22. doi: 10.1021/cb800120t. [Article]

Drug Relations

Drug Relations
DrugBank IDNameDrug groupPharmacological action?ActionsDetails
DB035937-methyl-5'-guanylic acidexperimentalunknownDetails