Analysis of the Escherichia coli genome. IV. DNA sequence of the region from 89.2 to 92.8 minutes.

Article Details


Blattner FR, Burland V, Plunkett G 3rd, Sofia HJ, Daniels DL

Analysis of the Escherichia coli genome. IV. DNA sequence of the region from 89.2 to 92.8 minutes.

Nucleic Acids Res. 1993 Nov 25;21(23):5408-17.

PubMed ID
8265357 [ View in PubMed

We present the sequence of 176 kilobases of the Escherichia coli K-12 genome, from katG at 89.2 to an open reading frame (ORF) of unknown function at 92.8 minutes on the genetic map. This brings the total of contiguous sequence from the E. coli genome project to 500 kb (81.5 to 92.8 minutes). This segment contains 134 putative coding genes (ORFs) of which 66 genes were previously identified. Eight new genes--acs, pepE, and nrfB-G--were identified as well as the previously mapped gldA and alr genes. Still, 58 ORFs remain unidentified despite literature and similarity searches. The arrangement of proposed genes relative to possible promoters and terminators suggests 55 potential transcription units. Other features include 13 REP elements, one IRU (ERIC) repeat, 59 computer-predicted bends, 42 Chi sites and one new grey hole. Sixteen signal peptides were found, including those of lamB, btuB, and malE. Two ribosomal RNA loci, rrnB and rrnE, are located in this segment, so we have now sequenced four of the seven E. coli rRNA loci. Comparison of the rRNA loci reveals some differences in the ribosomal structural RNAs which are generally compatible with the proposed secondary structures.

DrugBank Data that Cites this Article

NameUniProt ID
DNA-directed RNA polymerase subunit betaP0A8V2Details
Pantothenate kinaseP0A6I3Details
Formate dehydrogenase HP07658Details
Vitamin B12 transporter BtuBP06129Details
Chorismate pyruvate-lyaseP26602Details
Acetylglutamate kinaseP0A6C8Details
Methionine synthaseP13009Details
Phosphoenolpyruvate carboxylaseP00864Details
Maltose-binding periplasmic proteinP0AEX9Details
Class B acid phosphataseP0AE22Details
Cytochrome c-552P0ABK9Details
Single-stranded DNA-binding proteinP0AGE0Details
Hydrogen peroxide-inducible genes activatorP0ACQ4Details
UDP-N-acetylenolpyruvoylglucosamine reductaseP08373Details
Bifunctional ligase/repressor BirAP06709Details
DNA-directed RNA polymerase subunit beta'P0A8T7Details
UvrABC system protein AP0A698Details