A two-dimensional gel database of human colon carcinoma proteins.
Article Details
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Ji H, Reid GE, Moritz RL, Eddes JS, Burgess AW, Simpson RJ
A two-dimensional gel database of human colon carcinoma proteins.
Electrophoresis. 1997 Mar-Apr;18(3-4):605-13.
- PubMed ID
- 9150948 [ View in PubMed]
- Abstract
The master two-dimensional gel database of human colon carcinoma cells currently lists cellular proteins from normal crypts and the colorectal cancer cell lines LIM 1863, LIM 1215 and LIM 1899 (Ward et al., Electrophoresis 1990, 11, 883-891; Ji et al., Electrophoresis 1994, 15, 391-405). Updated two-dimensional electrophoretic (2-DE) maps of cellular proteins from LIM 1215 cells, acquired under both nonreducing and reducing conditions, are presented. Fifteen cellular proteins are identified in the reducing 2-DE gel map, and seven in the nonreducing gel map, along with a tabular listing of their M(r)/pI loci and mode of identification. We also include our mass spectrometric based procedures for identifying 2-DE resolved proteins. This procedure relies on a combination of capillary column (0.10-0.32 mm internal diameter) reversed-phase HPLC peptide mapping of in-gel digested proteins, peptide mass fingerprinting, sequence analysis by either collision-induced dissociation or post-source-decay fragmentation, and protein identification using available database search algorithms. These data, and descriptions of the micro-techniques employed in this laboratory for identifying 2-DE resolved proteins can be accessed via the internet URL: http:(/)/www.ludwig.edu.au.
DrugBank Data that Cites this Article
- Polypeptides
Name UniProt ID Argininosuccinate synthase P00966 Details Glutathione S-transferase P P09211 Details Endoplasmic reticulum chaperone BiP P11021 Details Heat shock protein HSP 90-beta P08238 Details Peroxiredoxin-2 P32119 Details Keratin, type II cytoskeletal 8 P05787 Details Calreticulin P27797 Details Protein disulfide-isomerase P07237 Details Triosephosphate isomerase P60174 Details Alpha-enolase P06733 Details 60 kDa heat shock protein, mitochondrial P10809 Details Protein disulfide-isomerase A3 P30101 Details Nucleophosmin P06748 Details