Penicillin-insensitive murein endopeptidase
Details
- Name
- Penicillin-insensitive murein endopeptidase
- Synonyms
- 3.4.24.-
- D-alanyl-D-alanine-endopeptidase
- DD-endopeptidase
- Gene Name
- mepA
- Organism
- Escherichia coli (strain K12)
- Amino acid sequence
>lcl|BSEQ0016643|Penicillin-insensitive murein endopeptidase MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGCIVGADTLPIQSEHYQV MRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPAGGRFNGGHASHQTGLDVD IFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEIFSLIKLAAQDKDVTRIFVNP AIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPADSLECEDQPLPPSGDGCGAELQ SWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI
- Number of residues
- 274
- Molecular Weight
- 30136.415
- Theoretical pI
- 8.4
- GO Classification
- Functionsendopeptidase activity / metal ion binding / metalloendopeptidase activity / peptidase activity / serine-type endopeptidase activityProcessespeptidoglycan biosynthetic process / peptidoglycan metabolic process / response to drugComponentsouter membrane-bounded periplasmic space
- General Function
- Serine-type endopeptidase activity
- Specific Function
- Involved in the removal of murein from the sacculus. May also facilitate integration of nascent murein strands into the sacculus by cleaving the peptide bonds between neighboring strands in mature murein.
- Pfam Domain Function
- Peptidase_M74 (PF03411)
- Transmembrane Regions
- Not Available
- Cellular Location
- Periplasm
- Gene sequence
>lcl|BSEQ0016644|Penicillin-insensitive murein endopeptidase (mepA) ATGAATAAAACCGCGATTGCGCTGCTGGCTCTGCTTGCCAGTAGCGCCAGCCTGGCAGCG ACGCCGTGGCAAAAAATAACCCAACCTGTGCCGGGTAGCGCACAATCGATAGGCAGTTTT TCTAATGGCTGTATTGTCGGCGCTGACACGCTGCCGATACAGTCCGAACATTATCAGGTC ATGCGTACCGATCAGCGTCGCTATTTCGGTCACCCGGATCTGGTGATGTTTATCCAGCGT CTGAGTAGCCAGGTGAGCAATCTGGGCATGGGTACGGTGCTGATTGGCGATATGGGGATG CCCGCTGGTGGGCGTTTCAACGGCGGTCATGCCAGCCACCAGACCGGACTGGATGTCGAT ATCTTTCTGCAACTGCCGAAAACTCGCTGGACCTCCGCGCAGCTCTTGCGCCCGCAAGCA CTGGACTTAGTTTCCCGCGACGGTAAACACGTTGTCTCCACGCTGTGGAAGCCAGAAATT TTCAGCTTGATCAAACTCGCCGCCCAGGACAAAGACGTCACGCGCATTTTTGTTAATCCG GCGATTAAACAACAACTTTGCCTTGATGCGGGCACCGATCGCGACTGGTTGCGCAAAGTG CGACCCTGGTTCCAGCATCGCGCGCATATGCATGTACGATTACGTTGCCCTGCCGATAGT CTGGAGTGTGAAGATCAACCTTTACCGCCATCAGGCGATGGTTGCGGGGCAGAACTGCAA AGCTGGTTTGAACCTCCAAAACCGGGAACAACAAAGCCTGAGAAGAAGACACCGCCTCCG TTGCCGCCTTCCTGCCAGGCGCTACTGGATGAGCACGTGATCTAA
- Chromosome Location
- Not Available
- Locus
- Not Available
- External Identifiers
Resource Link UniProtKB ID P0C0T5 UniProtKB Entry Name MEPA_ECOLI GenBank Protein ID 41993 GenBank Gene ID X16909 - General References
- Keck W, van Leeuwen AM, Huber M, Goodell EW: Cloning and characterization of mepA, the structural gene of the penicillin-insensitive murein endopeptidase from Escherichia coli. Mol Microbiol. 1990 Feb;4(2):209-19. [Article]
- Yamamoto Y, Aiba H, Baba T, Hayashi K, Inada T, Isono K, Itoh T, Kimura S, Kitagawa M, Makino K, Miki T, Mitsuhashi N, Mizobuchi K, Mori H, Nakade S, Nakamura Y, Nashimoto H, Oshima T, Oyama S, Saito N, Sampei G, Satoh Y, Sivasundaram S, Tagami H, Horiuchi T, et al.: Construction of a contiguous 874-kb sequence of the Escherichia coli -K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features. DNA Res. 1997 Apr 28;4(2):91-113. [Article]
- Blattner FR, Plunkett G 3rd, Bloch CA, Perna NT, Burland V, Riley M, Collado-Vides J, Glasner JD, Rode CK, Mayhew GF, Gregor J, Davis NW, Kirkpatrick HA, Goeden MA, Rose DJ, Mau B, Shao Y: The complete genome sequence of Escherichia coli K-12. Science. 1997 Sep 5;277(5331):1453-62. [Article]
- Hayashi K, Morooka N, Yamamoto Y, Fujita K, Isono K, Choi S, Ohtsubo E, Baba T, Wanner BL, Mori H, Horiuchi T: Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110. Mol Syst Biol. 2006;2:2006.0007. Epub 2006 Feb 21. [Article]
- Marcyjaniak M, Odintsov SG, Sabala I, Bochtler M: Peptidoglycan amidase MepA is a LAS metallopeptidase. J Biol Chem. 2004 Oct 15;279(42):43982-9. Epub 2004 Jul 29. [Article]