tRNA (cytidine(34)-2'-O)-methyltransferase

Details

Name

tRNA (cytidine(34)-2'-O)-methyltransferase

Synonyms

• 2.1.1.207
• tRNA (cytidine/uridine-2'-O-)-methyltransferase TrmL

Gene Name

trmL

Organism

Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)

Amino acid sequence

>lcl|BSEQ0011796|tRNA (cytidine(34)-2'-O)-methyltransferase
MLDIVLYEPEIPQNTGNIIRLCANTGFRLHLIEPLGFTWDDKRLRRSGLDYHEFAEIKRH
KTFEAFLESEKPKRLFALTTKGCPAHSQVKFKLGDYLMFGPETRGIPMSILNEMPMEQKI
RIPMTANSRSMNLSNSVAVTVYEAWRQLGYKGAVNLPEVK

Number of residues

160

Molecular Weight

18401.245

Theoretical pI

9.43

GO Classification

Functions

RNA binding / S-adenosylmethionine-dependent methyltransferase activity / tRNA methyltransferase activity

Components

cytoplasm

General Function

Trna methyltransferase activity

Specific Function

Methylates the ribose at the nucleotide 34 wobble position in the two leucyl isoacceptors tRNA(Leu)(CmAA) and tRNA(Leu)(cmnm5UmAA). Catalyzes the methyl transfer from S-adenosyl-L-methionine to the 2'-OH of the wobble nucleotide.

Pfam Domain Function

• SpoU_methylase (PF00588)

Transmembrane Regions

Not Available

Cellular Location

Cytoplasm

Gene sequence

>lcl|BSEQ0011797|tRNA (cytidine(34)-2'-O)-methyltransferase (trmL)
ATGTTAGATATCGTTTTATATGAACCAGAAATCCCACAAAATACTGGTAACATTATTCGA
CTTTGTGCCAACACAGGATTTCGCTTGCACTTAATTGAACCACTAGGTTTCACTTGGGAT
GATAAACGCTTACGCCGCTCAGGTTTGGATTATCACGAATTTGCGGAAATCAAACGCCAC
AAAACCTTTGAAGCCTTTTTAGAAAGCGAAAAACCCAAACGCCTTTTTGCACTTACAACA
AAAGGCTGTCCAGCACATAGCCAAGTGAAATTTAAACTTGGGGATTATTTAATGTTTGGC
CCAGAAACTCGTGGCATTCCGATGTCAATTTTAAATGAAATGCCGATGGAACAAAAAATC
CGCATTCCAATGACTGCCAATAGCCGCAGTATGAACTTGTCTAATTCTGTTGCAGTAACG
GTTTATGAAGCGTGGCGACAATTAGGCTATAAAGGTGCGGTAAATTTACCAGAAGTAAAA
TAA

Chromosome Location

Not Available

Locus

Not Available

External Identifiers

Resource	Link
UniProtKB ID	P44868
UniProtKB Entry Name	TRML_HAEIN
GenBank Gene ID	L42023

General References

1. Fleischmann RD, Adams MD, White O, Clayton RA, Kirkness EF, Kerlavage AR, Bult CJ, Tomb JF, Dougherty BA, Merrick JM, et al.: Whole-genome random sequencing and assembly of Haemophilus influenzae Rd. Science. 1995 Jul 28;269(5223):496-512. [Article]
2. Lim K, Zhang H, Tempczyk A, Krajewski W, Bonander N, Toedt J, Howard A, Eisenstein E, Herzberg O: Structure of the YibK methyltransferase from Haemophilus influenzae (HI0766): a cofactor bound at a site formed by a knot. Proteins. 2003 Apr 1;51(1):56-67. [Article]

Drug Relations

Drug Relations

DrugBank ID	Name	Drug group	Pharmacological action?	Actions	Details
DB01752	S-adenosyl-L-homocysteine	experimental	unknown		Details