Chromaffin granule amine transporter
Details
- Name
- Chromaffin granule amine transporter
- Synonyms
- Solute carrier family 18 member 1
- VAT1
- Vesicular amine transporter 1
- VMAT1
- Gene Name
- SLC18A1
- UniProtKB Entry
- P54219Swiss-Prot
- Organism
- Humans
- NCBI Taxonomy ID
- 9606
- Amino acid sequence
>lcl|BSEQ0009591|Chromaffin granule amine transporter MLRTILDAPQRLLKEGRASRQLVLVVVFVALLLDNMLFTVVVPIVPTFLYDMEFKEVNSS LHLGHAGSSPHALASPAFSTIFSFFNNNTVAVEESVPSGIAWMNDTASTIPPPATEAISA HKNNCLQGTGFLEEEITRVGVLFASKAVMQLLVNPFVGPLTNRIGYHIPMFAGFVIMFLS TVMFAFSGTYTLLFVARTLQGIGSSFSSVAGLGMLASVYTDDHERGRAMGTALGGLALGL LVGAPFGSVMYEFVGKSAPFLILAFLALLDGALQLCILQPSKVSPESAKGTPLFMLLKDP YILVAAGSICFANMGVAILEPTLPIWMMQTMCSPKWQLGLAFLPASVSYLIGTNLFGVLA NKMGRWLCSLIGMLVVGTSLLCVPLAHNIFGLIGPNAGLGLAIGMVDSSMMPIMGHLVDL RHTSVYGSVYAIADVAFCMGFAIGPSTGGAIVKAIGFPWLMVITGVINIVYAPLCYYLRS PPAKEEKLAILSQDCPMETRMYATQKPTKEFPLGEDSDEEPDHEE
- Number of residues
- 525
- Molecular Weight
- 56256.71
- Theoretical pI
- Not Available
- GO Classification
- Functionsmonoamine / serotonin / xenobiotic transmembrane transporter activityProcessesaminergic neurotransmitter loading into synaptic vesicle / dopamine uptake / norepinephrine uptakeComponentspresynapse / secretory granule membrane / synaptic vesicle membrane / terminal bouton
- General Function
- Electrogenic antiporter that exchanges one cationic monoamine with two intravesicular protons across the membrane of secretory and synaptic vesicles. Uses the electrochemical proton gradient established by the V-type proton-pump ATPase to accumulate high concentrations of monoamines inside the vesicles prior to their release via exocytosis. Transports catecholamines and indolamines with higher affinity for serotonin (PubMed:16326835, PubMed:23337945, PubMed:8643547). Regulates the transvesicular monoaminergic gradient that determines the quantal size. Mediates presynaptic monoaminergic vesicle transport in the amygdala and prefrontal brain regions related with emotion processing in response to environmental stimuli (PubMed:23337945)
- Specific Function
- monoamine transmembrane transporter activity
- Pfam Domain Function
- MFS_1 (PF07690)
- Signal Regions
- Not Available
- Transmembrane Regions
- 22-42 139-158 168-188 198-218 228-250 257-279 300-319 336-360 366-386 398-418 423-443 449-470
- Cellular Location
- Cytoplasmic vesicle, secretory vesicle membrane
- Gene sequence
>lcl|BSEQ0021405|Chromaffin granule amine transporter (SLC18A1) ATGCTCCGGACCATTCTGGATGCTCCCCAGCGGTTGCTGAAGGAGGGGAGAGCGTCCCGG CAGCTGGTGCTGGTGGTGGTATTCGTCGCTTTGCTCCTGGACAACATGCTGTTTACTGTG GTGGTGCCAATTGTGCCCACCTTCCTATATGACATGGAGTTCAAAGAAGTCAACTCTTCT CTGCACCTCGGCCATGCCGGAAGTTCCCCACATGCCCTCGCCTCTCCTGCCTTTTCCACC ATCTTCTCCTTCTTCAACAACAACACCGTGGCTGTTGAAGAAAGCGTACCTAGTGGAATA GCATGGATGAATGACACTGCCAGCACCATCCCACCTCCAGCCACTGAAGCCATCTCAGCT CATAAAAACAACTGCTTGCAAGGCACAGGTTTCTTGGAGGAAGAGATTACCCGGGTCGGG GTTCTGTTTGCTTCAAAGGCTGTGATGCAACTTCTGGTCAACCCATTCGTGGGCCCTCTC ACCAACAGGATTGGATATCATATCCCCATGTTTGCTGGCTTTGTTATCATGTTTCTCTCC ACAGTTATGTTTGCTTTTTCTGGGACCTATACTCTACTCTTTGTGGCCCGAACCCTTCAA GGCATTGGATCTTCATTTTCATCTGTTGCAGGTCTTGGAATGCTGGCCAGTGTCTACACT GATGACCATGAGAGAGGACGAGCCATGGGAACTGCTCTGGGGGGCCTGGCCTTGGGGTTG CTGGTGGGAGCTCCCTTTGGAAGTGTAATGTACGAGTTTGTTGGGAAGTCTGCACCCTTC CTCATCCTGGCCTTCCTGGCACTACTGGATGGAGCACTCCAGCTTTGCATCCTACAGCCT TCCAAAGTCTCTCCTGAGAGTGCCAAGGGGACTCCCCTCTTTATGCTTCTCAAAGACCCT TACATCCTGGTGGCTGCAGGGTCCATCTGCTTTGCCAACATGGGGGTGGCCATCCTGGAG CCCACACTGCCCATCTGGATGATGCAGACCATGTGCTCCCCCAAGTGGCAGCTGGGTCTA GCTTTCTTGCCTGCCAGTGTGTCCTACCTCATTGGCACCAACCTCTTTGGTGTGTTGGCC AACAAGATGGGTCGGTGGCTGTGTTCCCTAATCGGGATGCTGGTAGTAGGTACCAGCTTG CTCTGTGTTCCTCTGGCTCACAATATTTTTGGTCTCATTGGCCCCAATGCAGGGCTTGGC CTTGCCATAGGCATGGTGGATTCTTCTATGATGCCCATCATGGGGCACCTGGTGGATCTA CGCCACACCTCGGTGTATGGGAGTGTCTACGCCATCGCTGATGTGGCTTTTTGCATGGGC TTTGCTATAGGTCCATCCACCGGTGGTGCCATTGTAAAGGCCATCGGTTTTCCCTGGCTC ATGGTCATCACTGGGGTCATCAACATCGTCTATGCTCCACTCTGCTACTACCTGCGGAGC CCCCCGGCAAAGGAAGAGAAGCTTGCTATTCTGAGTCAGGACTGCCCCATGGAGACCCGG ATGTATGCAACCCAGAAGCCCACGAAGGAATTTCCTCTGGGGGAGGACAGTGATGAGGAG CCTGACCATGAGGAGTAG
- Chromosome Location
- 8
- Locus
- 8p21.3
- External Identifiers
Resource Link UniProtKB ID P54219 UniProtKB Entry Name VMAT1_HUMAN GeneCard ID SLC18A1 HGNC ID HGNC:10934 PDB ID(s) 8TGG, 8TGH, 8TGI, 8TGK, 8TGL, 8TGN KEGG ID hsa:6570 IUPHAR/Guide To Pharmacology ID 1011 NCBI Gene ID 6570 - General References
- Erickson JD, Schafer MK, Bonner TI, Eiden LE, Weihe E: Distinct pharmacological properties and distribution in neurons and endocrine cells of two isoforms of the human vesicular monoamine transporter. Proc Natl Acad Sci U S A. 1996 May 14;93(10):5166-71. [Article]
- Nusbaum C, Mikkelsen TS, Zody MC, Asakawa S, Taudien S, Garber M, Kodira CD, Schueler MG, Shimizu A, Whittaker CA, Chang JL, Cuomo CA, Dewar K, FitzGerald MG, Yang X, Allen NR, Anderson S, Asakawa T, Blechschmidt K, Bloom T, Borowsky ML, Butler J, Cook A, Corum B, DeArellano K, DeCaprio D, Dooley KT, Dorris L 3rd, Engels R, Glockner G, Hafez N, Hagopian DS, Hall JL, Ishikawa SK, Jaffe DB, Kamat A, Kudoh J, Lehmann R, Lokitsang T, Macdonald P, Major JE, Matthews CD, Mauceli E, Menzel U, Mihalev AH, Minoshima S, Murayama Y, Naylor JW, Nicol R, Nguyen C, O'Leary SB, O'Neill K, Parker SC, Polley A, Raymond CK, Reichwald K, Rodriguez J, Sasaki T, Schilhabel M, Siddiqui R, Smith CL, Sneddon TP, Talamas JA, Tenzin P, Topham K, Venkataraman V, Wen G, Yamazaki S, Young SK, Zeng Q, Zimmer AR, Rosenthal A, Birren BW, Platzer M, Shimizu N, Lander ES: DNA sequence and analysis of human chromosome 8. Nature. 2006 Jan 19;439(7074):331-5. [Article]
- Gerhard DS, Wagner L, Feingold EA, Shenmen CM, Grouse LH, Schuler G, Klein SL, Old S, Rasooly R, Good P, Guyer M, Peck AM, Derge JG, Lipman D, Collins FS, Jang W, Sherry S, Feolo M, Misquitta L, Lee E, Rotmistrovsky K, Greenhut SF, Schaefer CF, Buetow K, Bonner TI, Haussler D, Kent J, Kiekhaus M, Furey T, Brent M, Prange C, Schreiber K, Shapiro N, Bhat NK, Hopkins RF, Hsie F, Driscoll T, Soares MB, Casavant TL, Scheetz TE, Brown-stein MJ, Usdin TB, Toshiyuki S, Carninci P, Piao Y, Dudekula DB, Ko MS, Kawakami K, Suzuki Y, Sugano S, Gruber CE, Smith MR, Simmons B, Moore T, Waterman R, Johnson SL, Ruan Y, Wei CL, Mathavan S, Gunaratne PH, Wu J, Garcia AM, Hulyk SW, Fuh E, Yuan Y, Sneed A, Kowis C, Hodgson A, Muzny DM, McPherson J, Gibbs RA, Fahey J, Helton E, Ketteman M, Madan A, Rodrigues S, Sanchez A, Whiting M, Madari A, Young AC, Wetherby KD, Granite SJ, Kwong PN, Brinkley CP, Pearson RL, Bouffard GG, Blakesly RW, Green ED, Dickson MC, Rodriguez AC, Grimwood J, Schmutz J, Myers RM, Butterfield YS, Griffith M, Griffith OL, Krzywinski MI, Liao N, Morin R, Palmquist D, Petrescu AS, Skalska U, Smailus DE, Stott JM, Schnerch A, Schein JE, Jones SJ, Holt RA, Baross A, Marra MA, Clifton S, Makowski KA, Bosak S, Malek J: The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Genome Res. 2004 Oct;14(10B):2121-7. [Article]
- Essand M, Vikman S, Grawe J, Gedda L, Hellberg C, Oberg K, Totterman TH, Giandomenico V: Identification and characterization of a novel splicing variant of vesicular monoamine transporter 1. J Mol Endocrinol. 2005 Dec;35(3):489-501. [Article]
Associated Data
- Bio-Entities
Bio-Entity Type Chromaffin granule amine transporter (Humans) protein primary- Drug Relations
Drug Drug group Pharmacological action? Type Actions Details Ephedra sinica root nutraceutical yes target inhibitor Details MMDA experimental, illicit yes target inhibitor Details Metamfetamine approved, illicit, withdrawn yes target inhibitor Details Reserpine approved, investigational, withdrawn unknown target inhibitor Details Norepinephrine approved unknown target binder Details