Outer-membrane lipoprotein LolB

Details

Name
Outer-membrane lipoprotein LolB
Synonyms
  • hemM
  • ychC
Gene Name
lolB
UniProtKB Entry
P61320Swiss-Prot
Organism
Escherichia coli (strain K12)
NCBI Taxonomy ID
83333
Amino acid sequence
>lcl|BSEQ0012997|Outer-membrane lipoprotein LolB
MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQDVRNLNQYQTRGAFAY
ISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGNVQLVDNKGQRYTADDAEE
MIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEITYSQNGKNWKVVYGGYDTKT
QPAMPANMELTDGGQRIKLKMDNWIVK
Number of residues
207
Molecular Weight
23550.63
Theoretical pI
9.19
GO Classification
Functions
lipopeptide binding
Processes
lipid localization / lipoprotein localization to outer membrane / lipoprotein metabolic process / localization within membrane / protein transport
Components
cell outer membrane
General Function
Plays a critical role in the incorporation of lipoproteins in the outer membrane after they are released by the LolA protein. Essential for E.coli viability.
Specific Function
Lipopeptide binding
Pfam Domain Function
Signal Regions
1-21
Transmembrane Regions
Not Available
Cellular Location
Cell outer membrane
Gene sequence
>lcl|BSEQ0012998|Outer-membrane lipoprotein LolB (lolB)
ATGCCCCTGCCCGATTTTCGTCTTATCCGCCTGCTACCGCTGGCTGCTCTTGTGCTCACT
GCCTGTTCCGTTACCACGCCCAAAGGTCCTGGCAAAAGCCCGGATTCGCCACAATGGCGT
CAGCATCAGCAAGACGTGCGCAATCTTAATCAGTATCAGACTCGCGGCGCGTTCGCTTAT
ATTTCTGACCAACAAAAAGTGTACGCCCGCTTTTTCTGGCAGCAAACCGGCCAGGATCGC
TACCGTCTGCTGCTCACTAACCCATTGGGCAGCACGGAACTGGAGCTGAATGCTCAACCG
GGTAACGTGCAGTTAGTCGACAATAAAGGTCAGCGTTATACCGCCGATGACGCCGAAGAG
ATGATTGGCAAATTGACCGGAATGCCAATTCCGCTCAACAGCTTGCGCCAGTGGATTTTA
GGTTTACCGGGTGATGCAACCGACTACAAACTGGACGACCAGTACCGCCTGAGCGAAATT
ACCTACAGCCAGAATGGCAAAAACTGGAAGGTTGTTTATGGTGGTTATGACACCAAAACG
CAACCTGCGATGCCAGCCAATATGGAACTCACCGACGGTGGTCAACGCATCAAGTTAAAA
ATGGATAACTGGATAGTGAAATAA
Chromosome Location
Not Available
Locus
Not Available
External Identifiers
ResourceLink
UniProtKB IDP61320
UniProtKB Entry NameLOLB_ECOLI
GenBank Protein ID290462
GenBank Gene IDM77237
PDB ID(s)1IWM, 1IWN
KEGG IDecj:JW1200
NCBI Gene ID945775
General References
  1. Ikemi M, Murakami K, Hashimoto M, Murooka Y: Cloning and characterization of genes involved in the biosynthesis of delta-aminolevulinic acid in Escherichia coli. Gene. 1992 Nov 2;121(1):127-32. [Article]
  2. Post DA, Hove-Jensen B, Switzer RL: Characterization of the hemA-prs region of the Escherichia coli and Salmonella typhimurium chromosomes: identification of two open reading frames and implications for prs expression. J Gen Microbiol. 1993 Feb;139(2):259-66. [Article]
  3. Strohmaier H, Remler P, Renner W, Hogenauer G: Expression of genes kdsA and kdsB involved in 3-deoxy-D-manno-octulosonic acid metabolism and biosynthesis of enterobacterial lipopolysaccharide is growth phase regulated primarily at the transcriptional level in Escherichia coli K-12. J Bacteriol. 1995 Aug;177(15):4488-500. [Article]
  4. Oshima T, Aiba H, Baba T, Fujita K, Hayashi K, Honjo A, Ikemoto K, Inada T, Itoh T, Kajihara M, Kanai K, Kashimoto K, Kimura S, Kitagawa M, Makino K, Masuda S, Miki T, Mizobuchi K, Mori H, Motomura K, Nakamura Y, Nashimoto H, Nishio Y, Saito N, Horiuchi T, et al.: A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map. DNA Res. 1996 Jun 30;3(3):137-55. [Article]
  5. Blattner FR, Plunkett G 3rd, Bloch CA, Perna NT, Burland V, Riley M, Collado-Vides J, Glasner JD, Rode CK, Mayhew GF, Gregor J, Davis NW, Kirkpatrick HA, Goeden MA, Rose DJ, Mau B, Shao Y: The complete genome sequence of Escherichia coli K-12. Science. 1997 Sep 5;277(5331):1453-62. [Article]
  6. Hayashi K, Morooka N, Yamamoto Y, Fujita K, Isono K, Choi S, Ohtsubo E, Baba T, Wanner BL, Mori H, Horiuchi T: Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110. Mol Syst Biol. 2006;2:2006.0007. Epub 2006 Feb 21. [Article]
  7. Matsuyama Si, Yokota N, Tokuda H: A novel outer membrane lipoprotein, LolB (HemM), involved in the LolA (p20)-dependent localization of lipoproteins to the outer membrane of Escherichia coli. EMBO J. 1997 Dec 1;16(23):6947-55. [Article]
  8. Tanaka K, Matsuyama SI, Tokuda H: Deletion of lolB, encoding an outer membrane lipoprotein, is lethal for Escherichia coli and causes accumulation of lipoprotein localization intermediates in the periplasm. J Bacteriol. 2001 Nov;183(22):6538-42. [Article]
  9. Takeda K, Miyatake H, Yokota N, Matsuyama S, Tokuda H, Miki K: Crystal structures of bacterial lipoprotein localization factors, LolA and LolB. EMBO J. 2003 Jul 1;22(13):3199-209. [Article]

Associated Data

Bio-Entities
Bio-EntityType
Outer-membrane lipoprotein LolB (Escherichia coli (strain K12))protein
primary
Drug Relations
DrugDrug groupPharmacological action?TypeActionsDetails
TriglymeexperimentalunknowntargetDetails