Crystal structure of the ribosome at 5.5 A resolution.

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Yusupov MM, Yusupova GZ, Baucom A, Lieberman K, Earnest TN, Cate JH, Noller HF

Crystal structure of the ribosome at 5.5 A resolution.

Science. 2001 May 4;292(5518):883-96. Epub 2001 Mar 29.

PubMed ID
11283358 [ View in PubMed
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Abstract

We describe the crystal structure of the complete Thermus thermophilus 70S ribosome containing bound messenger RNA and transfer RNAs (tRNAs) at 5.5 angstrom resolution. All of the 16S, 23S, and 5S ribosomal RNA (rRNA) chains, the A-, P-, and E-site tRNAs, and most of the ribosomal proteins can be fitted to the electron density map. The core of the interface between the 30S small subunit and the 50S large subunit, where the tRNA substrates are bound, is dominated by RNA, with proteins located mainly at the periphery, consistent with ribosomal function being based on rRNA. In each of the three tRNA binding sites, the ribosome contacts all of the major elements of tRNA, providing an explanation for the conservation of tRNA structure. The tRNAs are closely juxtaposed with the intersubunit bridges, in a way that suggests coupling of the 20 to 50 angstrom movements associated with tRNA translocation with intersubunit movement.

DrugBank Data that Cites this Article

Polypeptides
NameUniProt ID
50S ribosomal protein L27P60493Details
30S ribosomal protein S10Q5SHN7Details
30S ribosomal protein S12Q5SHN3Details
30S ribosomal protein S11P80376Details
30S ribosomal protein S13P80377Details
30S ribosomal protein S15Q5SJ76Details
30S ribosomal protein S16Q5SJH3Details
30S ribosomal protein S18Q5SLQ0Details
30S ribosomal protein S19Q5SHP2Details
30S ribosomal protein S20P80380Details
30S ribosomal protein S4P80373Details
30S ribosomal protein S5Q5SHQ5Details
30S ribosomal protein S6Q5SLP8Details
30S ribosomal protein S7P17291Details
30S ribosomal protein S9P80374Details
30S ribosomal protein ThxQ5SIH3Details
30S ribosomal protein S2P80371Details
30S ribosomal protein S3P80372Details
30S ribosomal protein S14 type ZP0DOY6Details
30S ribosomal protein S17P0DOY7Details
30S ribosomal protein S8P0DOY9Details