Purification and characterization of the 30S ribosomal proteins from the bacterium Thermus thermophilus.

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Tsiboli P, Herfurth E, Choli T

Purification and characterization of the 30S ribosomal proteins from the bacterium Thermus thermophilus.

Eur J Biochem. 1994 Nov 15;226(1):169-77.

PubMed ID
7957245 [ View in PubMed
]
Abstract

The total protein mixture of the 30S subunit (TP-30) of the bacterium Thermus thermophilus has been purified using reverse-phase HPLC and the proteins obtained were identified both by means of two-dimensional polyacrylamide gel electrophoresis as well as by amino-terminal amino acid microsequence analysis. The proteins are numbered according to their primary structural similarity with known prokaryotic ribosomal proteins. Eight of them, namely proteins S6, S7, S9, S10, S14, S15, S16 and S17 run at different positions in the two-dimensional gel electrophoresis system to those suggested [Sedelnikova, S. C., Agalarov, M. B., Garber, M. & Yusupov, M. M. (1987) FEBS Lett. 220, 227-230]. All characterized proteins are homologous to known ribosomal proteins from other species, except for a small basic protein which shows homology only to a ribosomal protein from spinach chloroplasts [Choli, T., Franceschi, F., Yonath, A. & Wittmann-Leibold, B. (1993) Biol. Chem. Hoppe-Seyler 374, 377-383; Subramanian, A.-R. (1984) Trends Biochem. Sci. 9, 491-494].

DrugBank Data that Cites this Article

Polypeptides
NameUniProt ID
30S ribosomal protein S10Q5SHN7Details
30S ribosomal protein S12Q5SHN3Details
30S ribosomal protein S11P80376Details
30S ribosomal protein S13P80377Details
30S ribosomal protein S15Q5SJ76Details
30S ribosomal protein S16Q5SJH3Details
30S ribosomal protein S18Q5SLQ0Details
30S ribosomal protein S19Q5SHP2Details
30S ribosomal protein S20P80380Details
30S ribosomal protein S4P80373Details
30S ribosomal protein S5Q5SHQ5Details
30S ribosomal protein S6Q5SLP8Details
30S ribosomal protein S7P17291Details
30S ribosomal protein S9P80374Details
30S ribosomal protein ThxQ5SIH3Details
30S ribosomal protein S2P80371Details
30S ribosomal protein S3P80372Details
30S ribosomal protein S14 type ZP0DOY6Details
30S ribosomal protein S17P0DOY7Details
30S ribosomal protein S8P0DOY9Details