The path of messenger RNA through the ribosome.

Article Details

Citation
Copy to clipboard

Yusupova GZ, Yusupov MM, Cate JH, Noller HF

The path of messenger RNA through the ribosome.

Cell. 2001 Jul 27;106(2):233-41.

PubMed ID
11511350 [ View in PubMed
]
Abstract

Using X-ray crystallography, we have directly observed the path of mRNA in the 70S ribosome in Fourier difference maps at 7 A resolution. About 30 nucleotides of the mRNA are wrapped in a groove that encircles the neck of the 30S subunit. The Shine-Dalgarno helix is bound in a large cleft between the head and the back of the platform. At the interface, only about eight nucleotides (-1 to +7), centered on the junction between the A and P codons, are exposed, and bond almost exclusively to 16S rRNA. The mRNA enters the ribosome around position +13 to +15, the location of downstream pseudoknots that stimulate -1 translational frame shifting.

DrugBank Data that Cites this Article

Polypeptides
NameUniProt ID
30S ribosomal protein S10Q5SHN7Details
30S ribosomal protein S12Q5SHN3Details
30S ribosomal protein S11P80376Details
30S ribosomal protein S13P80377Details
30S ribosomal protein S15Q5SJ76Details
30S ribosomal protein S16Q5SJH3Details
30S ribosomal protein S18Q5SLQ0Details
30S ribosomal protein S19Q5SHP2Details
30S ribosomal protein S20P80380Details
30S ribosomal protein S4P80373Details
30S ribosomal protein S5Q5SHQ5Details
30S ribosomal protein S6Q5SLP8Details
30S ribosomal protein S7P17291Details
30S ribosomal protein S9P80374Details
30S ribosomal protein ThxQ5SIH3Details
30S ribosomal protein S2P80371Details
30S ribosomal protein S3P80372Details
30S ribosomal protein S14 type ZP0DOY6Details
30S ribosomal protein S17P0DOY7Details
30S ribosomal protein S8P0DOY9Details