Transport of the dopamine D2 agonist pramipexole by rat organic cation transporters OCT1 and OCT2 in kidney.

Article Details

Citation

Ishiguro N, Saito A, Yokoyama K, Morikawa M, Igarashi T, Tamai I

Transport of the dopamine D2 agonist pramipexole by rat organic cation transporters OCT1 and OCT2 in kidney.

Drug Metab Dispos. 2005 Apr;33(4):495-9. Epub 2005 Jan 7.

PubMed ID
15640376 [ View in PubMed
]
Abstract

We investigated the mechanism of renal tubular secretion of the dopamine D2 receptor agonist pramipexole in rats, focusing on organic cation transporters 1 and 2. The uptake of [14C]pramipexole by Xenopus oocytes injected with complementary RNA of either rat organic cation transporter (rOCT) 1 or rOCT2 was significantly higher than that by water-injected oocytes: the kinetic parameters, K(m) and V(max), of pramipexole uptake were 49.5 muM and 234 pmol/60 min/oocyte for rOCT1, and 16.9 microM and 12.8 pmol/60 min/oocyte for rOCT2. Pramipexole was taken up into kidney slices in a time- and concentration-dependent manner, and Eadie-Hofstee plots revealed the involvement of two saturable components. The kinetic parameters, K(m1) and V(max1), of the high-affinity component were 12.9 microM and 10.7 nmol/15 min/g kidney, respectively. The uptake of [14C]pramipexole by rOCT1, rOCT2, and kidney slices was inhibited by procainamide and corticosterone, which are selective inhibitors of rOCT1 and rOCT2, respectively. The IC50 values of procainamide and corticosterone for the uptake of [14C]pramipexole by rOCT1, rOCT2, and kidney slices were 7.7, 167.0, and 47.0 microM and 163.7, 10.7, and 47.7 microM, respectively. These results demonstrate that both rOCT1 and rOCT2 are involved in the renal uptake of pramipexole across the basolateral membrane of the proximal tubular epithelial cells.

DrugBank Data that Cites this Article

Drug Transporters
DrugTransporterKindOrganismPharmacological ActionActions
AmantadineSolute carrier family 22 member 2ProteinHumans
Unknown
Substrate
Inhibitor
Details
PramipexoleSolute carrier family 22 member 1ProteinHumans
Unknown
Substrate
Details
PramipexoleSolute carrier family 22 member 2ProteinHumans
Unknown
Substrate
Details
ProcainamideSolute carrier family 22 member 1ProteinHumans
Unknown
Inhibitor
Details
ProcainamideSolute carrier family 22 member 2ProteinHumans
Unknown
Inhibitor
Details
Drug Interactions
DrugsInteraction
Interactions
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